Detailed information of TA system TA240971

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1238259..1238827
• Replicon: chromosome
• Accession: NZ_CP095020
• Organism: Mycobacterium tuberculosis strain SGF0232017

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: MUW65_RS05935
• Protein ID: WP_003405865.1
• Coordinates: 1238453..1238827 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: MUW65_RS05930
• Protein ID: WP_003405863.1
• Coordinates: 1238259..1238456 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUW65_RS05910 (MUW65_05910)	1234300..1234938	-	639	WP_003898730.1	lipid droplet-associated protein	-
MUW65_RS05915 (MUW65_05915)	1235028..1236035	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
MUW65_RS05920 (MUW65_05920)	1236052..1237035	-	984	WP_003898731.1	hypothetical protein	-
MUW65_RS05925 (MUW65_05925)	1237098..1238171	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
MUW65_RS05930 (MUW65_05930)	1238259..1238456	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MUW65_RS05935 (MUW65_05935)	1238453..1238827	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
MUW65_RS05940 (MUW65_05940)	1239030..1239728	+	699	WP_003898733.1	hypothetical protein	-
MUW65_RS05945 (MUW65_05945)	1239846..1240031	+	186	WP_003901093.1	hypothetical protein	-
MUW65_RS05950 (MUW65_05950)	1239958..1240233	-	276	WP_003405867.1	hypothetical protein	-
MUW65_RS05955 (MUW65_05955)	1240476..1240799	+	324	WP_003405871.1	putative quinol monooxygenase	-
MUW65_RS05960 (MUW65_05960)	1240814..1241512	-	699	WP_031646953.1	hypothetical protein	-
MUW65_RS05965 (MUW65_05965)	1241546..1241755	+	210	WP_003911400.1	hypothetical protein	-
MUW65_RS05970 (MUW65_05970)	1241707..1242477	-	771	Protein_1175	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T240971 WP_003405865.1 NZ_CP095020:1238453-1238827 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T240971 NZ_LT900217:c52394-52029 [Gordonibacter urolithinfaciens]
ATGGGCTGGCATGTTGATATCGACTTGATCAAGCCTTGGCTCGATACCCAAGATCTCGTCACGGTTTCCTGCATAGAAGC
TGCGATCGAGGAACTTGAACGGAAGGGCCCGAGTCTCGGCAGGCCGCTGGTTGATCACATCAAGGACTCCGAGTTTTGCA
ATATGAAGGAGCTCCGTCCTGCATCGCCCGGGAGATCGGAAGTCCGCATTTTGTTTGCCTTCGATCCGAAAAGACATGCG
ATCATGCTTTTCGCCGGCGACAAATCGTCCGGAGGGAGAAGCCGCGAGAAATGGAACGGATGGTACCGATCGGCCATTCC
CTTGGCGGAAAAACGCTATCGCGAGCATCTAGGACGATTGGAGTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT240971 WP_003405863.1 NZ_CP095020:1238259-1238456 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT240971 NZ_LT900217:c52025-51723 [Gordonibacter urolithinfaciens]
ATGGACATTCACGAGTATGCTATCAAGCGCGGTGTCACCCAGGAAATGCGCGATCAAGCTCGCCGTGCTACGCAGGCTAA
GGTCGATGCGTACGACCTTAGCCAGGCGCGCAAGGAGCGCGATCTGACTCAAAGTGAGGTAGCAGCCAAGATGGGCGTCT
CCCAATGCCGCGTATCGGAGCTCGAGGCCGGTCGGCTCGCCACGATGCGCGTCGATACACTGTCGCGCTACATTTCGAGC
CTTGGTGGTAAGCTCGTTATCAGCGCTGAATGGCCCGACAAGACCATCCCTTTGACCATCTAA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07