Detailed information of TA system TA240968

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1013423..1014042
• Replicon: chromosome
• Accession: NZ_CP095020
• Organism: Mycobacterium tuberculosis strain SGF0232017

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: MUW65_RS04805
• Protein ID: WP_003404726.1
• Coordinates: 1013608..1014042 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: MUW65_RS04800
• Protein ID: WP_003898641.1
• Coordinates: 1013423..1013602 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUW65_RS04785 (MUW65_04785)	1008896..1010476	+	1581	WP_003910977.1	serine hydrolase	-
MUW65_RS04790 (MUW65_04790)	1010473..1012866	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
MUW65_RS04795 (MUW65_04795)	1013139..1013297	+	159	WP_003404720.1	hypothetical protein	-
MUW65_RS04800 (MUW65_04800)	1013423..1013602	+	180	WP_003898641.1	antitoxin	Antitoxin
MUW65_RS04805 (MUW65_04805)	1013608..1014042	+	435	WP_003404726.1	SRPBCC family protein	Toxin
MUW65_RS04810 (MUW65_04810)	1014140..1014913	+	774	WP_003404735.1	VOC family protein	-
MUW65_RS04815 (MUW65_04815)	1014978..1015427	+	450	WP_003404738.1	hypothetical protein	-
MUW65_RS04820 (MUW65_04820)	1015562..1015792	-	231	WP_003898642.1	hypothetical protein	-
MUW65_RS04825 (MUW65_04825)	1015959..1017467	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
MUW65_RS04830 (MUW65_04830)	1017469..1018707	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T240968 WP_003404726.1 NZ_CP095020:1013608-1014042 [Mycobacterium tuberculosis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T240968 NZ_LT900021:90859-91245 [Thermococcus henrietii]
ATGACGGTCATAGACACCAACGTTTTTCTTTATGCGGTTCTGAAGGACTCTGAACTTAACGAGGAGGCGAGGAACCTGCT
GGCGTCACTGGAGCGATGGGTCGTTCCGAGCATGGTTCTCTACGAGCTCTACTGGTTTTTAAAGAAGAGGGAGTACTCGG
TGGAAGACATCAACGGGGTAATCTCCGCGGTTCTCTCAAGTCCCAGAACGAAGGTCGTCGGCGACAACGGGAAATACACG
AAGGAAGCCCTGAAGCTGACCAAGAACCCCAAGCGGTTCAACGACATGGTAATCCTCGCAACCGCGAAGGACTTTGGAAG
GCTCGCAACCTACGATAAAAAGCTGAGAAAAGAGGCGGAAAAACTCGGAATCAAAGTGCTACCCTAA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT240968 WP_003898641.1 NZ_CP095020:1013423-1013602 [Mycobacterium tuberculosis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT240968 NZ_LT900021:90638-90862 [Thermococcus henrietii]
ATGAGCCTGACAAAGGTAACTAGGAACTACCAAATTACTATACCAAGCGAGATTAGGAAAAAGCTTGGGATTAAAGTGGG
GGACATACTGCTCGTTGAGGTTGAGGACGGGAAGGTTGTTCTCAAAAAGAGCGAGCTTGAGCTTCCACTGCTTCCGGGTG
GAAAGAAGCTGACTGTTGAGGAAATCGAAGAAACAATCATCGAGGGCATCGGTGACGAAGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5