Detailed information of TA system TA240959

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 701755..702398
• Replicon: chromosome
• Accession: NZ_CP095020
• Organism: Mycobacterium tuberculosis strain SGF0232017

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P67239
• Locus tag: MUW65_RS03185
• Protein ID: WP_003403187.1
• Coordinates: 701997..702398 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ91
• Locus tag: MUW65_RS03180
• Protein ID: WP_003403184.1
• Coordinates: 701755..702000 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUW65_RS03145 (MUW65_03145)	697035..697565	-	531	WP_003917320.1	two component system sensor kinase HK2	-
MUW65_RS03150 (MUW65_03150)	697549..698244	-	696	WP_003918099.1	two-component system response regulator TcrA	-
MUW65_RS03155 (MUW65_03155)	698367..698678	+	312	WP_003403164.1	hypothetical protein	-
MUW65_RS03160 (MUW65_03160)	698750..699700	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
MUW65_RS03165 (MUW65_03165)	699941..700525	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
MUW65_RS03170 (MUW65_03170)	700527..701237	+	711	Protein_628	IS607 family element RNA-guided endonuclease TnpB	-
MUW65_RS03175 (MUW65_03175)	701240..701710	+	471	WP_003898523.1	hypothetical protein	-
MUW65_RS03180 (MUW65_03180)	701755..702000	+	246	WP_003403184.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MUW65_RS03185 (MUW65_03185)	701997..702398	+	402	WP_003403187.1	type II toxin-antitoxin system VapC family toxin	Toxin
MUW65_RS03190 (MUW65_03190)	702389..702568	+	180	Protein_632	hypothetical protein	-
MUW65_RS03195 (MUW65_03195)	702986..703183	-	198	WP_003403191.1	hypothetical protein	-
MUW65_RS03200 (MUW65_03200)	703263..704420	-	1158	WP_003898524.1	hypothetical protein	-
MUW65_RS03205 (MUW65_03205)	704472..704693	-	222	WP_003898525.1	DUF4926 domain-containing protein	-
MUW65_RS03210 (MUW65_03210)	704835..705440	+	606	WP_003898526.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-80)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14501.21 Da
• Isoelectric Point: 4.4463

>T240959 WP_003403187.1 NZ_CP095020:701997-702398 [Mycobacterium tuberculosis]
VIVDTSAIIAILRDEDDAAAYADALANADVRRLSAASYLECGIVLDSQRDPVISRALDELIEEAEFVVEPVTERQARLAR
AAYADFGRGSGHPAGLNFGDCLSYALAIDRREPLLWKGNDFGHTGVQRALDRR

Nucleotide

• Length: 402 bp

>T240959 NZ_LT897798:c483425-483156 [Vibrio cholerae]
ATGTATAAACTCGAATACTCCACACAGTTTAAGAAAGATTTCAAAAAGATAACTAAGATGCCGATTTCAGACATCATTGA
AGTCGGCAATGTTATTTCTAAGCTGCAACGCGGCGAAAAGCTTGAACCCAAGAATGTTGACCATCCGTTAACTGGAAATT
GGGTTGGATTTCGAGACTGCCACATTAAACCCGACCTAGTGTTAATCTATCGAGTTTTTAACGATCAACTACAACTAGCG
CGCATTGGTTCACATAGTGATTTATTCTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8813.12 Da
• Isoelectric Point: 10.3486

>AT240959 WP_003403184.1 NZ_CP095020:701755-702000 [Mycobacterium tuberculosis]
MALNIKDPSVHQAVKQIAKITGESQARAVATAVNERLARLRSDDLAARLLAIGHKTASRMSPEAKRLDHDALLYDERGLP
A

Nucleotide

• Length: 246 bp

>AT240959 NZ_LT897798:c483696-483418 [Vibrio cholerae]
ATGAGAACAGAAATGCTGAGCACGCGCATTGACCACGACACGAAAATTGCGTTCACAAACGTCTGCGATGAGATGGGTCT
AAGTACATCACAGGCCATAAAGTTATTTGCAAAAGCGGTTATTAATCATGGTGGAATCCCTTTTGAGCTGCGAGTTCAAC
AGCCTAATGAGGTTACTGCATCTGCAATTCAAGAGCTCGTTGAAGGAAAAGGACATAAAGCTGAATCCGTTGAAGCTATG
CTGAATGAGCTTACTGAAGGCAAAGTTAAGCATGTATAA

Structures

Toxin

• AlphaFold DB: A0A7U4BSD6

Antitoxin

• AlphaFold DB: G0TQ91