Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 693350..693996 Replicon chromosome
Accession NZ_CP095020
Organism Mycobacterium tuberculosis strain SGF0232017

Toxin (Protein)


Gene name vapC Uniprot ID O07783
Locus tag MUW65_RS03115 Protein ID WP_003403122.1
Coordinates 693350..693742 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TQ86
Locus tag MUW65_RS03120 Protein ID WP_003403125.1
Coordinates 693739..693996 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUW65_RS03100 (MUW65_03100) 689012..690538 + 1527 WP_003403112.1 virulence factor Mce family protein -
MUW65_RS03105 (MUW65_03105) 690601..691743 + 1143 WP_003911253.1 virulence factor Mce family protein -
MUW65_RS03110 (MUW65_03110) 691748..693298 + 1551 WP_003905349.1 MCE family protein -
MUW65_RS03115 (MUW65_03115) 693350..693742 - 393 WP_003403122.1 type II toxin-antitoxin system toxin ribonuclease C4 Toxin
MUW65_RS03120 (MUW65_03120) 693739..693996 - 258 WP_003403125.1 type II toxin-antitoxin system antitoxin VapB4 Antitoxin
MUW65_RS03125 (MUW65_03125) 694179..695414 - 1236 WP_003403128.1 ATP-binding protein -
MUW65_RS03130 (MUW65_03130) 695665..696078 - 414 WP_003403137.1 type II toxin-antitoxin system VapC family toxin -
MUW65_RS03135 (MUW65_03135) 696075..696311 - 237 WP_003403139.1 type II toxin-antitoxin system antitoxin VapB27 -
MUW65_RS03140 (MUW65_03140) 696415..696921 - 507 WP_003900973.1 two component system sensor kinase HK1 -
MUW65_RS03145 (MUW65_03145) 697035..697565 - 531 WP_003917320.1 two component system sensor kinase HK2 -
MUW65_RS03150 (MUW65_03150) 697549..698244 - 696 WP_003918099.1 two-component system response regulator TcrA -
MUW65_RS03155 (MUW65_03155) 698367..698678 + 312 WP_003403164.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(8-122)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(3-53)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14080.08 Da        Isoelectric Point: 4.6558

>T240957 WP_003403122.1 NZ_CP095020:c693742-693350 [Mycobacterium tuberculosis]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI

Download         Length: 393 bp

>T240957 NZ_LT897798:443369-443635 [Vibrio cholerae]
ATGATTAAATTTGAATTTGATGAAAACAAAAGCAGAAGTAATCTCGAAAAGCACGGTATTGATTTTCATACAGCTCAAGG
GTTGTGGAATGATTCAGATCTTATCGAGATCCCTGCTAACACGAGTGATGAGCCCAGATATTTGGTTGTCGGCTTACTAA
ACGGTAAGCATTGGTCAGGAGTAATTACCTACCGAGGTACGAATATCAGGATCATCTCGGTTCGGCGTTCACGGAAAGCG
GAGGTAAGCCTTTATGAAAGCGCATGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9681.06 Da        Isoelectric Point: 7.0175

>AT240957 WP_003403125.1 NZ_CP095020:c693996-693739 [Mycobacterium tuberculosis]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Download         Length: 258 bp

>AT240957 NZ_LT897798:443622-443867 [Vibrio cholerae]
ATGAAAGCGCATGAATTTGATGCCAAGTTTGAAAGTGACGACGATGATATCGTAATGGACTTGGATCTATCGCAAGCTAA
AAGACCGATGCATAAGCAAAAACGAGTCAATGTAGATTTTCCAGCCTGGATGCTTGAGTCCTTGGACAGAGAAGCGAGTC
GTATTGGTGTAACACGTCAATCAATCATTAAAATTTGGCTGGCAGAAAGGTTAGAGAGCGTGTCTCATCATTCAAGTGTG
AGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F8V4

Antitoxin

Source ID Structure
AlphaFold DB G0TQ86

References