Detailed information of TA system TA240955

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 638744..639420
• Replicon: chromosome
• Accession: NZ_CP095020
• Organism: Mycobacterium tuberculosis strain SGF0232017

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TPQ9
• Locus tag: MUW65_RS02880
• Protein ID: WP_003898500.1
• Coordinates: 638744..639157 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TPR0
• Locus tag: MUW65_RS02885
• Protein ID: WP_003402915.1
• Coordinates: 639154..639420 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUW65_RS02850 (MUW65_02850)	634089..634391	-	303	WP_003402896.1	DUF3349 domain-containing protein	-
MUW65_RS02855 (MUW65_02855)	634451..634729	-	279	WP_003402901.1	hypothetical protein	-
MUW65_RS02860 (MUW65_02860)	634726..635979	-	1254	WP_003402904.1	inorganic phosphate transporter	-
MUW65_RS02865 (MUW65_02865)	636099..636485	-	387	WP_003402907.1	VOC family protein	-
MUW65_RS02870 (MUW65_02870)	636548..637432	-	885	WP_003402909.1	SDR family oxidoreductase	-
MUW65_RS02875 (MUW65_02875)	637528..638430	-	903	WP_003915439.1	1,4-dihydroxy-2-naphthoyl-CoA synthase	-
MUW65_RS02880 (MUW65_02880)	638744..639157	-	414	WP_003898500.1	type II toxin-antitoxin system VapC family toxin	Toxin
MUW65_RS02885 (MUW65_02885)	639154..639420	-	267	WP_003402915.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
MUW65_RS02890 (MUW65_02890)	639612..641327	-	1716	WP_003402917.1	fatty-acid--CoA ligase FadD8	-
MUW65_RS02895 (MUW65_02895)	641405..643009	+	1605	WP_003402920.1	amidohydrolase	-
MUW65_RS02900 (MUW65_02900)	643006..643986	+	981	WP_003402923.1	o-succinylbenzoate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (13-129)

Antitoxin

• Post-segregation antitoxin CcdA (19-61)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14667.78 Da
• Isoelectric Point: 7.3603

>T240955 WP_003898500.1 NZ_CP095020:c639157-638744 [Mycobacterium tuberculosis]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Nucleotide

• Length: 414 bp

>T240955 NZ_LT897798:439151-439627 [Vibrio cholerae]
ATGAACTTAGAAGAGTTTCAAGAATCGGATTTTGACCTGCTTATAAAATGGATTGACTCAGATGAGTTGAACTATTTATG
GGGTGGCCCTGCGTATGTTTTCCCGCTGACTTATGAACAGATCCATTCCCACTGTAGCAAAGCGGAAGTATTTCCCTATC
TGCTAAATGTGAACGGTAGACATGCCGGGTTTGTTGAGTTGTACAAAGTCACCGATGAGCAATATCGGATTTGCCGTGTT
TTTATTTCAAATGCTTATCGTGGGCAAGGGCTATCAAAATCGATGTTGATGTTATTAATCGATAAAGCCCGATTAGATTT
TTCTGCAACCAAGCTTAGCTTAGGCGTTTTTGAACAGAACACAGTTGCTAGAAAATGCTATGAGTCATTAGGTTTTGAAG
TGGTTTCGACAGAAATTGGTACAAGAGCTTTTAATGGTAAGTTATGGGATCTGGTGCGAATGGAAAAACGGTTATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9543.79 Da
• Isoelectric Point: 4.5994

>AT240955 WP_003402915.1 NZ_CP095020:c639420-639154 [Mycobacterium tuberculosis]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Nucleotide

• Length: 267 bp

>AT240955 NZ_LT897798:438696-439013 [Vibrio cholerae]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTGAGTAATTTGATTAATGGCGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCAGCGGCACTAGGTAATACACCAGAGTTTTGGCTTAATATTCAACATGCAGTTGATCTTTGGGAC
ACGAGAAATCGTTATCAAGAAGAAGCAAAATTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Structures

Toxin

• AlphaFold DB: G0TPQ9

Antitoxin

• AlphaFold DB: G0TPR0