Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 73147..73780 Replicon chromosome
Accession NZ_CP095020
Organism Mycobacterium tuberculosis strain SGF0232017

Toxin (Protein)


Gene name vapC Uniprot ID P9WFC0
Locus tag MUW65_RS00370 Protein ID WP_003400580.1
Coordinates 73379..73780 (+) Length 134 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P0CW29
Locus tag MUW65_RS00365 Protein ID WP_003400577.1
Coordinates 73147..73386 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUW65_RS00350 (MUW65_00350) 68443..69883 + 1441 Protein_66 FAD-binding oxidoreductase -
MUW65_RS00355 (MUW65_00355) 69939..70100 + 162 WP_003899796.1 hypothetical protein -
MUW65_RS00360 (MUW65_00360) 70141..73080 + 2940 WP_003901785.1 UPF0182 family protein -
MUW65_RS00365 (MUW65_00365) 73147..73386 + 240 WP_003400577.1 antitoxin VapB1 Antitoxin
MUW65_RS00370 (MUW65_00370) 73379..73780 + 402 WP_003400580.1 type II toxin-antitoxin system ribonuclease VapC1 Toxin
MUW65_RS00375 (MUW65_00375) 73832..76069 - 2238 WP_003899797.1 NADP-dependent isocitrate dehydrogenase -
MUW65_RS00380 (MUW65_00380) 76187..76756 - 570 WP_003400591.1 TetR/AcrR family transcriptional regulator -
MUW65_RS00385 (MUW65_00385) 76859..77770 + 912 WP_003899798.1 SDR family NAD(P)-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Pae0151-like, PIN domain

(36-124)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14339.44 Da        Isoelectric Point: 4.8887

>T240950 WP_003400580.1 NZ_CP095020:73379-73780 [Mycobacterium tuberculosis]
VDECVVDAAAVVDALAGKGASAIVLRGLLKESISNAPHLLDAEVGHALRRAVLSDEISEEQARAALDALPYLIDNRYPHS
PRLIEYTWQLRHNVTFYDALYVALATALDVPLLTGDSRLAAAPGLPCEIKLVR

Download         Length: 402 bp

>T240950 NZ_LT883143:c5597591-5597181 [Pseudomonas aeruginosa C-NN2]
ATGCTCAAGTTCATGCTCGACACCAACATCTGCATCTTCACCATCAAAAACCGCCCGCAAGAGGTGCGCGAGGCGTTCCT
GCGCCACCACGATCAGATGTGCATCAGCACCGTGACGCTGATGGAGCTGATCTACGGCGCAGAGAAGTCCTCGAACCCCT
CGCGCAATCTTGCAGACGTGGAAGGCTTCGCTGCTCGTCTGGAGGTGCTCAAGTACGACCAGGAAGCTGCCGCCCACACC
GGCCAGCTCCGCGCTGAACTGGCCCGCCTCGGCAAGCAAATCGGCCCCTATGACCAGATGATTGCCGGGCACGCCCGCTC
TCAGGGCCTGATCGTGGTCACCAATAACCGCCGCGAGTTCGACCGCGTACCCGGCTTGAGAGTTGAGGACTGGGTCGTCC
CAATCGTGTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 8717.84 Da        Isoelectric Point: 8.5960

>AT240950 WP_003400577.1 NZ_CP095020:73147-73386 [Mycobacterium tuberculosis]
MATIQVRDLPEDVAETYRRRATAAGQSLQTYMRTKLIEGVRGRDKAEAIEILEQALASTASPGISRETIEASRRELRGG

Download         Length: 240 bp

>AT240950 NZ_LT883143:c5597821-5597591 [Pseudomonas aeruginosa C-NN2]
ATGGAACAAGGTTCTGTTTTTCAGAGCAATCGTAGCCAGGCCGTAAGACTGCCCAAGGCAGTCGCGCTTCCCGATGACGT
TAAGCGTGTGGATGTGGTCGTTGTGGGGCGCGCCAGAATCATCACGCCTGCCGGCGAGTCTTGGGATAGCTGGTTCGATG
GCGAGGGCGTTACTGCCGACTTTATGGCAGAGCGTGAACAGCCTGCCGATCAGGAGCGCGAGACTTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BR82

Antitoxin

Source ID Structure
AlphaFold DB A0A829CHM7

References