Detailed information of TA system TA240944

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 76119..76641
• Replicon: plasmid pE_NDM
• Accession: NZ_CP095004
• Organism: Klebsiella michiganensis strain fxq

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A7H0EVH6
• Locus tag: MT472_RS33030
• Protein ID: WP_014839901.1
• Coordinates: 76119..76403 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A7H0EVH5
• Locus tag: MT472_RS33035
• Protein ID: WP_014839900.1
• Coordinates: 76393..76641 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MT472_RS32995 (MT472_32995)	71714..72442	+	729	WP_042934505.1	thioredoxin fold domain-containing protein	-
MT472_RS33000 (MT472_33000)	72459..72764	+	306	WP_014839905.1	hypothetical protein	-
MT472_RS33005 (MT472_33005)	72778..73119	+	342	WP_042934503.1	hypothetical protein	-
MT472_RS33010 (MT472_33010)	73183..73392	+	210	WP_125316032.1	hypothetical protein	-
MT472_RS33015 (MT472_33015)	73455..74399	+	945	WP_014839903.1	DsbA family protein	-
MT472_RS33020 (MT472_33020)	74495..75475	+	981	WP_032202633.1	IS5-like element ISKpn26 family transposase	-
MT472_RS33025 (MT472_33025)	75584..75928	-	345	WP_244673380.1	hypothetical protein	-
MT472_RS33030 (MT472_33030)	76119..76403	-	285	WP_014839901.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MT472_RS33035 (MT472_33035)	76393..76641	-	249	WP_014839900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MT472_RS33040 (MT472_33040)	76982..78385	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
MT472_RS33045 (MT472_33045)	78414..79046	-	633	WP_001567369.1	hypothetical protein	-
MT472_RS33050 (MT472_33050)	79265..80668	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
MT472_RS33055 (MT472_33055)	80697..81328	-	632	Protein_93	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / aac(3)-IId / sul1 / blaDHA-1 / qnrB4 / blaNDM-1 / qacE / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr / qnrS1	-	1..255357	255357
	-	inside	IScluster/Tn	-	-	74495..80668	6173

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10866.46 Da
• Isoelectric Point: 10.5831

>T240944 WP_014839901.1 NZ_CP095004:c76403-76119 [Klebsiella michiganensis]
MTYKLAFNESALKEWKKLGHTIREQFKKKLSERLQNPRVPAAQLHGGKDQYKIKLHGAGYRLVYSVNDEIVTVTVIGVGK
RNNDDIYNATRHRN

Nucleotide

• Length: 285 bp

>T240944 NZ_LT883142:c4257282-4257109 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9071.29 Da
• Isoelectric Point: 3.8832

>AT240944 WP_014839900.1 NZ_CP095004:c76641-76393 [Klebsiella michiganensis]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMEMLEDQELGRIADERIGEDRIEVNID
DL

Nucleotide

• Length: 249 bp

>AT240944 NZ_LT883142:c4257606-4257310 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A7H0EVH6

Antitoxin

• AlphaFold DB: A0A7H0EVH5