Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 6158..6801 Replicon plasmid pD
Accession NZ_CP095003
Organism Klebsiella michiganensis strain fxq

Toxin (Protein)


Gene name vagD Uniprot ID A0A5P6KDG5
Locus tag MT472_RS32055 Protein ID WP_021312768.1
Coordinates 6385..6801 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A5P6KGW8
Locus tag MT472_RS32050 Protein ID WP_021312767.1
Coordinates 6158..6388 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MT472_RS32015 (MT472_32015) 1471..2097 + 627 WP_016946792.1 ParA family plasmid-partitioning AAA ATPase -
MT472_RS32020 (MT472_32020) 2094..2396 + 303 WP_004197636.1 hypothetical protein -
MT472_RS32025 (MT472_32025) 2804..3598 - 795 WP_016240356.1 site-specific integrase -
MT472_RS32030 (MT472_32030) 3796..4811 - 1016 Protein_4 hypothetical protein -
MT472_RS32035 (MT472_32035) 5005..5310 - 306 WP_006788214.1 type II toxin-antitoxin system toxin CcdB -
MT472_RS32040 (MT472_32040) 5312..5530 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA -
MT472_RS32045 (MT472_32045) 5700..6104 - 405 Protein_7 hypothetical protein -
MT472_RS32050 (MT472_32050) 6158..6388 + 231 WP_021312767.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MT472_RS32055 (MT472_32055) 6385..6801 + 417 WP_021312768.1 type II toxin-antitoxin system VapC family toxin Toxin
MT472_RS32060 (MT472_32060) 6989..7693 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
MT472_RS32065 (MT472_32065) 7758..10532 + 2775 Protein_11 Tn3 family transposase -
MT472_RS32070 (MT472_32070) 10740..11708 - 969 WP_077255425.1 IS5-like element IS903B family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..107094 107094


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15175.60 Da        Isoelectric Point: 7.2452

>T240943 WP_021312768.1 NZ_CP095003:6385-6801 [Klebsiella michiganensis]
VNKIYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPDLVLEDWVR

Download         Length: 417 bp

>T240943 NZ_LT883142:3701414-3701692 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8722.81 Da        Isoelectric Point: 4.4692

>AT240943 WP_021312767.1 NZ_CP095003:6158-6388 [Klebsiella michiganensis]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT240943 NZ_LT883142:3701151-3701411 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5P6KDG5

Antitoxin

Source ID Structure
AlphaFold DB A0A5P6KGW8

References