Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5005..5530 Replicon plasmid pD
Accession NZ_CP095003
Organism Klebsiella michiganensis strain fxq

Toxin (Protein)


Gene name ccdB Uniprot ID A0A1D8K3Q5
Locus tag MT472_RS32035 Protein ID WP_006788214.1
Coordinates 5005..5310 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag MT472_RS32040 Protein ID WP_001568025.1
Coordinates 5312..5530 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MT472_RS32010 (MT472_32010) 19..894 + 876 WP_004098982.1 replication initiation protein -
MT472_RS32015 (MT472_32015) 1471..2097 + 627 WP_016946792.1 ParA family plasmid-partitioning AAA ATPase -
MT472_RS32020 (MT472_32020) 2094..2396 + 303 WP_004197636.1 hypothetical protein -
MT472_RS32025 (MT472_32025) 2804..3598 - 795 WP_016240356.1 site-specific integrase -
MT472_RS32030 (MT472_32030) 3796..4811 - 1016 Protein_4 hypothetical protein -
MT472_RS32035 (MT472_32035) 5005..5310 - 306 WP_006788214.1 type II toxin-antitoxin system toxin CcdB Toxin
MT472_RS32040 (MT472_32040) 5312..5530 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
MT472_RS32045 (MT472_32045) 5700..6104 - 405 Protein_7 hypothetical protein -
MT472_RS32050 (MT472_32050) 6158..6388 + 231 WP_021312767.1 type II toxin-antitoxin system VapB family antitoxin -
MT472_RS32055 (MT472_32055) 6385..6801 + 417 WP_021312768.1 type II toxin-antitoxin system VapC family toxin -
MT472_RS32060 (MT472_32060) 6989..7693 - 705 WP_001067855.1 IS6-like element IS26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..107094 107094


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11687.46 Da        Isoelectric Point: 7.2015

>T240942 WP_006788214.1 NZ_CP095003:c5310-5005 [Klebsiella michiganensis]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRLMTTDMASVPASVTGEEV
ADLRHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T240942 NZ_LT883142:c3695360-3694962 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGCGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT240942 WP_001568025.1 NZ_CP095003:c5530-5312 [Klebsiella michiganensis]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT240942 NZ_LT883142:c3695656-3695363 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D8K3Q5

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References