Detailed information of TA system TA240941

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 64314..64849
• Replicon: plasmid pB_KPC
• Accession: NZ_CP095001
• Organism: Klebsiella michiganensis strain fxq

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A6M4NRI2
• Locus tag: MT472_RS30890
• Protein ID: WP_032693963.1
• Coordinates: 64314..64601 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: MT472_RS30895
• Protein ID: WP_032693964.1
• Coordinates: 64598..64849 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MT472_RS30860 (MT472_30860)	59904..60536	+	633	WP_080884176.1	ParA family protein	-
MT472_RS30865 (MT472_30865)	60536..60910	+	375	WP_088903436.1	hypothetical protein	-
MT472_RS30870 (MT472_30870)	61150..62124	+	975	WP_080884175.1	plasmid segregation protein ParM	-
MT472_RS30875 (MT472_30875)	62128..62520	+	393	WP_080884174.1	plasmid partitioning/stability family protein	-
MT472_RS30880 (MT472_30880)	62796..63296	-	501	WP_080884173.1	PBECR2 nuclease fold domain-containing protein	-
MT472_RS30885 (MT472_30885)	64043..64231	-	189	WP_080884172.1	hypothetical protein	-
MT472_RS30890 (MT472_30890)	64314..64601	-	288	WP_032693963.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MT472_RS30895 (MT472_30895)	64598..64849	-	252	WP_032693964.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MT472_RS30900 (MT472_30900)	65016..65990	-	975	Protein_69	translesion error-prone DNA polymerase V subunit UmuC	-
MT472_RS30905 (MT472_30905)	66420..67853	-	1434	WP_088903432.1	multidrug efflux transporter outer membrane subunit TOprJ2	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2	-	1..140266	140266

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11133.12 Da
• Isoelectric Point: 10.2246

>T240941 WP_032693963.1 NZ_CP095001:c64601-64314 [Klebsiella michiganensis]
MTYTVKFREDALKEWQKLDKAIQQQFAKKLKKCCDEPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDDQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T240941 NZ_LT883142:3487305-3487523 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9202.49 Da
• Isoelectric Point: 4.0785

>AT240941 WP_032693964.1 NZ_CP095001:c64849-64598 [Klebsiella michiganensis]
MPNIILSDTSASISELKKNPMATVSAGDGFPVAILNRNQPAFYCIPAELYEKMLDALDDQELAKLVSERSNQRLLDVDLD
TYL

Nucleotide

• Length: 252 bp

>AT240941 NZ_LT883142:3486905-3487279 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A6M4NRI2