Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 5426278..5427082 Replicon chromosome
Accession NZ_CP094999
Organism Klebsiella michiganensis strain fxq

Toxin (Protein)


Gene name yeeV Uniprot ID A0A2J4ZCW3
Locus tag MT472_RS26025 Protein ID WP_042944193.1
Coordinates 5426278..5426667 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A2J4ZCX4
Locus tag MT472_RS26030 Protein ID WP_042944192.1
Coordinates 5426723..5427082 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MT472_RS25995 (MT472_25995) 5421331..5422125 + 795 WP_038424010.1 DNA/RNA non-specific endonuclease -
MT472_RS26000 (MT472_26000) 5422415..5422738 + 324 WP_032747696.1 endoribonuclease SymE -
MT472_RS26005 (MT472_26005) 5422885..5423319 + 435 WP_004098173.1 VOC family protein -
MT472_RS26010 (MT472_26010) 5423485..5424228 + 744 WP_014227721.1 hypothetical protein -
MT472_RS26015 (MT472_26015) 5424406..5425203 - 798 WP_042944196.1 helix-turn-helix transcriptional regulator -
MT472_RS26020 (MT472_26020) 5425346..5426191 - 846 WP_042944195.1 DUF4942 domain-containing protein -
MT472_RS26025 (MT472_26025) 5426278..5426667 - 390 WP_042944193.1 TA system toxin CbtA family protein Toxin
MT472_RS26030 (MT472_26030) 5426723..5427082 - 360 WP_042944192.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
MT472_RS26035 (MT472_26035) 5427107..5427328 - 222 WP_004192297.1 DUF987 domain-containing protein -
MT472_RS26040 (MT472_26040) 5427342..5427821 - 480 WP_042944191.1 DNA repair protein RadC -
MT472_RS26045 (MT472_26045) 5427833..5428276 - 444 WP_042944189.1 antirestriction protein -
MT472_RS26050 (MT472_26050) 5428356..5428586 - 231 WP_227666078.1 DUF905 domain-containing protein -
MT472_RS26055 (MT472_26055) 5428610..5429434 - 825 WP_042944188.1 DUF932 domain-containing protein -
MT472_RS26060 (MT472_26060) 5429977..5430609 + 633 WP_049100008.1 inovirus Gp2 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 5422885..5462146 39261


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(3-111)

Antitoxin

Antitoxin CbeA

(15-114)


Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 14418.54 Da        Isoelectric Point: 8.9846

>T240939 WP_042944193.1 NZ_CP094999:c5426667-5426278 [Klebsiella michiganensis]
MQTKSPPFMRAASSRPSPVGVWQTLLTCLLEHHYGLTLSDTPFSDEQVIQQHIDAGISLVDALNFIVEKYELVRTDRPGF
SILTQSPFITPIDILRARKATGLMNRDTYKEVTAITRGQHPQVSAPGKR

Download         Length: 390 bp

>T240939 NZ_LT883142:2676183-2676290 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13305.20 Da        Isoelectric Point: 7.0166

>AT240939 WP_042944192.1 NZ_CP094999:c5427082-5426723 [Klebsiella michiganensis]
MSKNTTITTHDISEPWWGLRRSVSPCFGARLVQEGNRLHYLADRANVSGQFCDADLRHLDQAFPVLMKQLELMLTCGELN
PRHQHCVTLYAKGLTCEADTLGSHGYVYIAIYPTPVATA

Download         Length: 360 bp

>AT240939 NZ_LT883142:c2676136-2676070 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZCW3

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZCX4

References