Detailed information of TA system TA240938

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 5370221..5370797
• Replicon: chromosome
• Accession: NZ_CP094999
• Organism: Klebsiella michiganensis strain fxq

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: MT472_RS25765
• Protein ID: WP_038424021.1
• Coordinates: 5370510..5370797 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A0H3H591
• Locus tag: MT472_RS25760
• Protein ID: WP_014227757.1
• Coordinates: 5370221..5370523 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MT472_RS25745 (MT472_25745)	5367061..5367396	+	336	WP_014227760.1	endoribonuclease SymE	-
MT472_RS25750 (MT472_25750)	5367849..5368760	+	912	WP_032719936.1	acetamidase/formamidase family protein	-
MT472_RS25755 (MT472_25755)	5368757..5370100	+	1344	WP_032719935.1	APC family permease	-
MT472_RS25760 (MT472_25760)	5370221..5370523	-	303	WP_014227757.1	BrnA antitoxin family protein	Antitoxin
MT472_RS25765 (MT472_25765)	5370510..5370797	-	288	WP_038424021.1	BrnT family toxin	Toxin
MT472_RS25770 (MT472_25770)	5371047..5371490	-	444	WP_014837312.1	FosA family fosfomycin resistance glutathione transferase	-
MT472_RS25775 (MT472_25775)	5371484..5372392	-	909	WP_014837311.1	LysR family transcriptional regulator	-
MT472_RS25780 (MT472_25780)	5372480..5373262	+	783	WP_014837310.1	NAD(P)H-dependent oxidoreductase	-
MT472_RS25785 (MT472_25785)	5373410..5373994	+	585	WP_004098242.1	TetR/AcrR family transcriptional regulator	-
MT472_RS25790 (MT472_25790)	5374140..5374940	+	801	WP_014227752.1	winged helix-turn-helix domain-containing protein	-
MT472_RS25795 (MT472_25795)	5374937..5375455	+	519	WP_042944214.1	FidL-like protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (38-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11253.72 Da
• Isoelectric Point: 8.5899

>T240938 WP_038424021.1 NZ_CP094999:c5370797-5370510 [Klebsiella michiganensis]
MPMEFEWDANKARSNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTLGLVHGIIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Nucleotide

• Length: 288 bp

>T240938 NZ_LT883142:c2418558-2418382 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11607.18 Da
• Isoelectric Point: 9.4429

>AT240938 WP_014227757.1 NZ_CP094999:c5370523-5370221 [Klebsiella michiganensis]
MNMVKHKRDNPDTLTAKRDDELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Nucleotide

• Length: 303 bp

>AT240938 NZ_LT883142:c2418336-2417920 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3H591