Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4644933..4645552 Replicon chromosome
Accession NZ_CP094999
Organism Klebsiella michiganensis strain fxq

Toxin (Protein)


Gene name Hha Uniprot ID H3N9D8
Locus tag MT472_RS22345 Protein ID WP_004099646.1
Coordinates 4645334..4645552 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID H3N7X7
Locus tag MT472_RS22340 Protein ID WP_004129911.1
Coordinates 4644933..4645307 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MT472_RS22330 (MT472_22330) 4640090..4641283 + 1194 WP_042944463.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MT472_RS22335 (MT472_22335) 4641306..4644452 + 3147 WP_014228207.1 multidrug efflux RND transporter permease subunit AcrB -
MT472_RS22340 (MT472_22340) 4644933..4645307 + 375 WP_004129911.1 Hha toxicity modulator TomB Antitoxin
MT472_RS22345 (MT472_22345) 4645334..4645552 + 219 WP_004099646.1 HHA domain-containing protein Toxin
MT472_RS22350 (MT472_22350) 4645715..4646281 + 567 WP_042944461.1 maltose O-acetyltransferase -
MT472_RS22355 (MT472_22355) 4646253..4646387 - 135 WP_223226764.1 hypothetical protein -
MT472_RS22360 (MT472_22360) 4646408..4646878 + 471 WP_014228205.1 YlaC family protein -
MT472_RS22365 (MT472_22365) 4646853..4648307 - 1455 WP_042944459.1 PLP-dependent aminotransferase family protein -
MT472_RS22370 (MT472_22370) 4648409..4649107 + 699 WP_014228203.1 GNAT family protein -
MT472_RS22375 (MT472_22375) 4649104..4649244 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
MT472_RS22380 (MT472_22380) 4649244..4649507 - 264 WP_004848323.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8640.05 Da        Isoelectric Point: 8.9008

>T240935 WP_004099646.1 NZ_CP094999:4645334-4645552 [Klebsiella michiganensis]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Download         Length: 219 bp

>T240935 NZ_LT883142:1960930-1961033 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14350.09 Da        Isoelectric Point: 4.8989

>AT240935 WP_004129911.1 NZ_CP094999:4644933-4645307 [Klebsiella michiganensis]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT240935 NZ_LT883142:c1961071-1960800 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H713

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HD25

References