Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2263571..2264161 Replicon chromosome
Accession NZ_CP094999
Organism Klebsiella michiganensis strain fxq

Toxin (Protein)


Gene name pemK Uniprot ID A0A0H3HDW1
Locus tag MT472_RS11145 Protein ID WP_014229979.1
Coordinates 2263829..2264161 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID J6I3K7
Locus tag MT472_RS11140 Protein ID WP_004852307.1
Coordinates 2263571..2263828 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MT472_RS11115 (MT472_11115) 2258827..2259432 - 606 WP_042945602.1 glutathione S-transferase family protein -
MT472_RS11120 (MT472_11120) 2259612..2260538 + 927 WP_042945600.1 LysR substrate-binding domain-containing protein -
MT472_RS11125 (MT472_11125) 2260576..2261574 - 999 WP_014229982.1 aldo/keto reductase -
MT472_RS11130 (MT472_11130) 2261675..2262589 + 915 WP_042945598.1 LysR family transcriptional regulator -
MT472_RS11135 (MT472_11135) 2262780..2262860 - 81 Protein_2185 molybdopterin-binding protein -
MT472_RS11140 (MT472_11140) 2263571..2263828 + 258 WP_004852307.1 antitoxin Antitoxin
MT472_RS11145 (MT472_11145) 2263829..2264161 + 333 WP_014229979.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MT472_RS11155 (MT472_11155) 2264484..2265941 + 1458 WP_014229916.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
MT472_RS11165 (MT472_11165) 2266904..2268358 - 1455 WP_014229909.1 AMP nucleosidase -
MT472_RS11170 (MT472_11170) 2268491..2268748 - 258 WP_014229908.1 histidine kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11740.57 Da        Isoelectric Point: 10.1863

>T240926 WP_014229979.1 NZ_CP094999:2263829-2264161 [Klebsiella michiganensis]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVSLEGAGTKTTGVIRCDQPRTI
DMAARNGKRLESIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T240926 NZ_LT883142:55206-55313 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9422.88 Da        Isoelectric Point: 4.4607

>AT240926 WP_004852307.1 NZ_CP094999:2263571-2263828 [Klebsiella michiganensis]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT240926 NZ_LT883142:c55157-55094 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3HDW1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HDW8

References