Detailed information of TA system TA240924

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 929183..929840
• Replicon: chromosome
• Accession: NZ_CP094999
• Organism: Klebsiella michiganensis strain fxq

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: MT472_RS04570
• Protein ID: WP_042946033.1
• Coordinates: 929430..929840 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: H3N295
• Locus tag: MT472_RS04565
• Protein ID: WP_004124953.1
• Coordinates: 929183..929449 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MT472_RS04550 (MT472_04550)	924417..924842	-	426	WP_032720093.1	PTS sugar transporter subunit IIA	-
MT472_RS04555 (MT472_04555)	924963..927761	-	2799	WP_014226794.1	transcriptional regulator DagR	-
MT472_RS04560 (MT472_04560)	927955..928938	-	984	WP_032720095.1	tRNA-modifying protein YgfZ	-
MT472_RS04565 (MT472_04565)	929183..929449	+	267	WP_004124953.1	FAD assembly factor SdhE	Antitoxin
MT472_RS04570 (MT472_04570)	929430..929840	+	411	WP_042946033.1	protein YgfX	Toxin
MT472_RS04575 (MT472_04575)	929849..930370	-	522	WP_014226792.1	flavodoxin FldB	-
MT472_RS04580 (MT472_04580)	930492..931388	+	897	WP_004105555.1	site-specific tyrosine recombinase XerD	-
MT472_RS04585 (MT472_04585)	931411..932124	+	714	WP_004854054.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
MT472_RS04590 (MT472_04590)	932130..933863	+	1734	WP_038423602.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16137.00 Da
• Isoelectric Point: 10.9455

>T240924 WP_042946033.1 NZ_CP094999:929430-929840 [Klebsiella michiganensis]
VVLWQSDLRISWRAQWFSLLMHGVVATLVLLMPWPLSYTPLWLILLSLVVFDCVRSQRRIHARQGEIKLLIDSRLRWQKA
EWDIIGTPWVINSGMLLRLRNTENQRTQHLWVAADSMDAGEWRDLRRLVLLKPTQE

Nucleotide

• Length: 411 bp

>T240924 NZ_LT883139:84522-84902 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTCCAGGCCAGAGTTGTGTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACTGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCGGAGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10411.88 Da
• Isoelectric Point: 5.1296

>AT240924 WP_004124953.1 NZ_CP094999:929183-929449 [Klebsiella michiganensis]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDTLSDGDKALFIRLLENDDPDLFNWLMNHGEPADAELQRMVKLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT240924 NZ_LT883139:84301-84522 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3H3L9