Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 17449..18092 Replicon plasmid pB-8919_KPC-2
Accession NZ_CP094994
Organism Klebsiella pneumoniae strain SCPM-O-B-8919 (12PKP/19c)

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag KF986_RS28945 Protein ID WP_001044770.1
Coordinates 17676..18092 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag KF986_RS28940 Protein ID WP_001261282.1
Coordinates 17449..17679 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KF986_RS28900 (KF986_028900) 12647..13120 + 474 WP_004152341.1 YkgJ family cysteine cluster protein -
KF986_RS28905 (KF986_028905) 13212..13442 + 231 WP_011977773.1 hypothetical protein -
KF986_RS28910 (KF986_028910) 14334..15116 - 783 WP_004152340.1 site-specific integrase -
KF986_RS28915 (KF986_028915) 15116..15448 - 333 WP_004152339.1 hypothetical protein -
KF986_RS28920 (KF986_028920) 15455..15853 - 399 WP_004171440.1 hypothetical protein -
KF986_RS28925 (KF986_028925) 15879..16208 - 330 WP_004152337.1 hypothetical protein -
KF986_RS28930 (KF986_028930) 16236..16544 - 309 WP_004152336.1 hypothetical protein -
KF986_RS28935 (KF986_028935) 17031..17492 - 462 WP_072093212.1 hypothetical protein -
KF986_RS28940 (KF986_028940) 17449..17679 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KF986_RS28945 (KF986_028945) 17676..18092 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
KF986_RS28950 (KF986_028950) 18166..18855 + 690 Protein_19 AAA family ATPase -
KF986_RS28955 (KF986_028955) 18910..19607 + 698 WP_223174630.1 IS1 family transposase -
KF986_RS28960 (KF986_028960) 19671..20081 + 411 WP_004152401.1 hypothetical protein -
KF986_RS28965 (KF986_028965) 20738..21097 - 360 WP_117046906.1 DUF305 domain-containing protein -
KF986_RS28970 (KF986_028970) 21269..21499 - 231 Protein_23 IS5/IS1182 family transposase -
KF986_RS28975 (KF986_028975) 21601..22785 + 1185 WP_004152398.1 ISAs1-like element ISKpn31 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 - 1..102252 102252
- flank IS/Tn - - 11259..12527 1268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T240910 WP_001044770.1 NZ_CP094994:17676-18092 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T240910 NZ_LT854705:1376857-1377114 [Levilactobacillus zymae]
ATGGGAATTTATTGGTCAGACGACGCTTGGCAAGATTACAGGTACTGGCAAGACCAGGGTGACAAGAAAAAGCTCCGCAA
AATCAATGCGCTGATTAAAGACATTACTCGTCACCCTTTTTCGGGACAAGGTAAGCCGGAACCTCTCAAACATGAGCTGA
CCGGCAAGTGGTCGCGGCGGATTACCGGCGAGTATCGGTTGATTTATCGGATCGAAGATGACGCGTTATATTTATATTCT
GCCCGTGATTATTATTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT240910 WP_001261282.1 NZ_CP094994:17449-17679 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT240910 NZ_LT854705:1376589-1376855 [Levilactobacillus zymae]
ATGGACGCTGTTAACTACACAAATTTTCGGCAAAACCTCAAATCCTTTATGAAGACGGTCAATGAAGACTCCGAACCACT
GATCGTCACCACTAAAAAAGGAGAAGATGACGTTGTGGTTTTGTCTAAAGACGATTACGATGCGATGACCGAGACCATGC
GCATTCTTTCGAACCAACCCCTAATGGACAAGATTAGGCGCGGGGATGCCCAATTTGCCAAGGGGCAAGGCAAACAACAT
GCCCTCATAGACCCCGAGGATGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References