Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4082307..4082804 Replicon chromosome
Accession NC_012947
Organism Escherichia coli BL21-Gold(DE3)pLysS AG

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag ECBD_RS20400 Protein ID WP_001173343.1
Coordinates 4082307..4082480 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag ECBD_RS20405 Protein ID WP_000398619.1
Coordinates 4082508..4082804 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECBD_RS20390 4078977..4080434 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
ECBD_RS20395 4080671..4082188 + 1518 WP_001295090.1 lysine--tRNA ligase -
ECBD_RS20400 4082307..4082480 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
ECBD_RS20405 4082508..4082804 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
ECBD_RS20410 4083031..4083303 - 273 WP_000405651.1 N-acetyltransferase -
ECBD_RS20415 4083315..4083545 - 231 WP_001070602.1 (4Fe-4S)-binding protein -
ECBD_RS20420 4083726..4085362 + 1637 Protein_3892 sensor histidine kinase -
ECBD_RS20425 4085359..4086078 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T24091 WP_001173343.1 NC_012947:c4082480-4082307 [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T24091 NC_012947:c4082480-4082307 [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT24091 WP_000398619.1 NC_012947:c4082804-4082508 [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT24091 NC_012947:c4082804-4082508 [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References