Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vagCD/VapC-VagC |
| Location | 281645..282315 | Replicon | plasmid pB-8919_OXA-48 |
| Accession | NZ_CP094992 | ||
| Organism | Klebsiella pneumoniae strain SCPM-O-B-8919 (12PKP/19c) | ||
Toxin (Protein)
| Gene name | vagD | Uniprot ID | Q6U619 |
| Locus tag | KF986_RS27610 | Protein ID | WP_004213072.1 |
| Coordinates | 281645..282088 (-) | Length | 148 a.a. |
Antitoxin (Protein)
| Gene name | vagC | Uniprot ID | Q6U620 |
| Locus tag | KF986_RS27615 | Protein ID | WP_004213073.1 |
| Coordinates | 282085..282315 (-) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KF986_RS27580 (KF986_027580) | 277481..277884 | + | 404 | Protein_317 | GAF domain-containing protein | - |
| KF986_RS27585 (KF986_027585) | 278402..278757 | - | 356 | Protein_318 | mucoid phenotype regulator RmpA2 | - |
| KF986_RS27590 (KF986_027590) | 278987..280183 | + | 1197 | WP_249012456.1 | IS110-like element ISEc20 family transposase | - |
| KF986_RS27595 (KF986_027595) | 280217..280501 | - | 285 | Protein_320 | mucoid phenotype regulator RmpA2 | - |
| KF986_RS27600 (KF986_027600) | 280918..281222 | + | 305 | Protein_321 | transposase | - |
| KF986_RS27605 (KF986_027605) | 281245..281496 | - | 252 | WP_186987481.1 | hypothetical protein | - |
| KF986_RS27610 (KF986_027610) | 281645..282088 | - | 444 | WP_004213072.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| KF986_RS27615 (KF986_027615) | 282085..282315 | - | 231 | WP_004213073.1 | type II toxin-antitoxin system VapB family antitoxin | Antitoxin |
| KF986_RS27620 (KF986_027620) | 282923..284056 | + | 1134 | WP_004213075.1 | DUF3800 domain-containing protein | - |
| KF986_RS27625 (KF986_027625) | 284072..284365 | + | 294 | WP_004213076.1 | hypothetical protein | - |
| KF986_RS27630 (KF986_027630) | 284355..284561 | - | 207 | WP_004213077.1 | hypothetical protein | - |
| KF986_RS27635 (KF986_027635) | 284913..285203 | + | 291 | WP_004213078.1 | hypothetical protein | - |
| KF986_RS27640 (KF986_027640) | 285193..286092 | + | 900 | WP_004225022.1 | nucleotide-binding protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | blaOXA-48 / catA1 / blaOXA-1 / aac(6')-Ib-cr / blaTEM-1B / qnrS1 / blaCTX-M-15 / ant(2'')-Ia / ant(3'')-Ia / qacE / sul1 | iucA / iucB / iucC / iucD / iutA / rmpA2 | 1..326663 | 326663 | |
| - | flank | IS/Tn | - | - | 278987..280183 | 1196 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 148 a.a. Molecular weight: 16006.74 Da Isoelectric Point: 9.3642
>T240908 WP_004213072.1 NZ_CP094992:c282088-281645 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS
Download Length: 444 bp
>T240908 NZ_LT853886:c11531-11238 [Xanthomonas fragariae]
GTGCTCGTTCTTGAGTGGCGCGAGACAGCCCGCGCCGATCTGCTGGCGATTGTCGATTACATATCCGACGACAACCCGGA
TGCAGCGCAGCGGTTGAAAGATGACATCGAGGCCAAGGCCTCGATGCTGCCTGAGCGCCCGAAGCTGTATCGGCCTGGTC
GCGTCGCCGGCACTCGGGAAATGGTCGTCCGATCGAACTACGTGGTGGTGTACGCGGAAGACGCGCGCGCCGTTTCCATC
CTGCGGGTGCTGCATGCGGCCCAACAGTGGCCGCCAGTGACCGGGGTTGAGTGA
GTGCTCGTTCTTGAGTGGCGCGAGACAGCCCGCGCCGATCTGCTGGCGATTGTCGATTACATATCCGACGACAACCCGGA
TGCAGCGCAGCGGTTGAAAGATGACATCGAGGCCAAGGCCTCGATGCTGCCTGAGCGCCCGAAGCTGTATCGGCCTGGTC
GCGTCGCCGGCACTCGGGAAATGGTCGTCCGATCGAACTACGTGGTGGTGTACGCGGAAGACGCGCGCGCCGTTTCCATC
CTGCGGGTGCTGCATGCGGCCCAACAGTGGCCGCCAGTGACCGGGGTTGAGTGA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8677.73 Da Isoelectric Point: 4.4563
>AT240908 WP_004213073.1 NZ_CP094992:c282315-282085 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP
Download Length: 231 bp
>AT240908 NZ_LT853886:c11817-11518 [Xanthomonas fragariae]
ATGGCTGCTCACACCTCGATGCTGCATATCCGCGTCGATGACGCTCTAAAGGCACAGGCTGCCGAGACGCTCGCCAATGT
CGGCCTGACGGTCTCGGATGCTGTGCGCATCCTGCTGACCCGCGTCGCCAAGGAAGGCGGGTTGCCAGCCGGCCTGACGG
CCGATCCAGAGGCCCACGACGCCTGGTTCCGGGGCAAGGTGCAAGAGGCCCTGGCCGACACGCGGCCGGCGGTGCCGCAT
CGCCAGGTGATGGATGAGGCGCAAGCCCTGATCGACCGGAAACGCCGTGCTCGTTCTTGA
ATGGCTGCTCACACCTCGATGCTGCATATCCGCGTCGATGACGCTCTAAAGGCACAGGCTGCCGAGACGCTCGCCAATGT
CGGCCTGACGGTCTCGGATGCTGTGCGCATCCTGCTGACCCGCGTCGCCAAGGAAGGCGGGTTGCCAGCCGGCCTGACGG
CCGATCCAGAGGCCCACGACGCCTGGTTCCGGGGCAAGGTGCAAGAGGCCCTGGCCGACACGCGGCCGGCGGTGCCGCAT
CGCCAGGTGATGGATGAGGCGCAAGCCCTGATCGACCGGAAACGCCGTGCTCGTTCTTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|