Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 148899..149809 Replicon plasmid pB-8919_OXA-48
Accession NZ_CP094992
Organism Klebsiella pneumoniae strain SCPM-O-B-8919 (12PKP/19c)

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag KF986_RS26900 Protein ID WP_004026354.1
Coordinates 149339..149809 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag KF986_RS26895 Protein ID WP_004026357.1
Coordinates 148899..149342 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KF986_RS26870 (KF986_026870) 144055..144270 - 216 Protein_174 IS200/IS605 family transposase -
KF986_RS26875 (KF986_026875) 144272..144841 + 570 Protein_175 transposase -
KF986_RS26880 (KF986_026880) 145209..146696 - 1488 WP_004178082.1 group II intron reverse transcriptase/maturase -
KF986_RS26885 (KF986_026885) 147106..147528 + 423 WP_004181893.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
KF986_RS26890 (KF986_026890) 147528..148799 + 1272 WP_004181894.1 translesion error-prone DNA polymerase V subunit UmuC -
KF986_RS26895 (KF986_026895) 148899..149342 + 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
KF986_RS26900 (KF986_026900) 149339..149809 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
KF986_RS26905 (KF986_026905) 149918..150178 - 261 WP_004026352.1 hypothetical protein -
KF986_RS26915 (KF986_026915) 151205..151864 + 660 WP_000412211.1 type A-1 chloramphenicol O-acetyltransferase -
KF986_RS26920 (KF986_026920) 152065..152442 - 378 WP_000656305.1 GNAT family N-acetyltransferase -
KF986_RS26925 (KF986_026925) 152509..153342 - 834 Protein_185 Tn3 family transposase -
KF986_RS26930 (KF986_026930) 153407..154111 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
KF986_RS26935 (KF986_026935) 154165..154605 - 441 Protein_187 CatB-related O-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaOXA-48 / catA1 / blaOXA-1 / aac(6')-Ib-cr / blaTEM-1B / qnrS1 / blaCTX-M-15 / ant(2'')-Ia / ant(3'')-Ia / qacE / sul1 iucA / iucB / iucC / iucD / iutA / rmpA2 1..326663 326663
- flank IS/Tn - - 144488..144841 353


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T240907 WP_004026354.1 NZ_CP094992:149339-149809 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T240907 NZ_LT853885:c4197495-4197214 [Xanthomonas fragariae]
ATGATCAAGAGCATTGTCGACAAGGAAGCCGAAAAGATCTGGGTGGGTGAGCGCTCCCGCCGGTTGCCGGCCGACATCCA
GTCGGTCGCGCGCCGCAAGTTGCGCATGCTCAACGCTGCCGCGCACCTCGACGATCTGCGCATTCCGCCTGCCAACCGGC
TGGAAGCGTTGAAGGGCGAGCGGCGCGGGCAGTACAGCATCCGGATCAACGACCAGTTGCGCATCTGCTTCCGATGGATG
GAGGGCGATGTCGCCGAGGTCGAAATTGTCGATTACCACTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT240907 WP_004026357.1 NZ_CP094992:148899-149342 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT240907 NZ_LT853885:c4197195-4196905 [Xanthomonas fragariae]
ATGACAGTCCTACCCAACATCCACCCTGGTGAAATCCTGCTTGAGGAGTTCCTCGAGCCGATGGGCATCAGCCAGAACGC
ATTGGCGCGGGCCACCGGTGTGCCGCCGCGCCGTATCAACGAAATCGTGTTGGGCAAGCGCGGCATCACCGCCGACACCG
CCGTGCGCCTGGCTGCAGCGCTGTGCACGACCGAGCGCTTCTGGCTAGGCCTGCAAGCCGATTACGAGCTGGAACAAGCG
CACCGCGCGCTGGGCGACCTGCCATCGCGGATCAAGCGGCTGGCGGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References