Detailed information of TA system TA240906

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-DnaT
• Location: 45174..45696
• Replicon: plasmid pB-8919_OXA-48
• Accession: NZ_CP094992
• Organism: Klebsiella pneumoniae strain SCPM-O-B-8919 (12PKP/19c)

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A2J4R0S6
• Locus tag: KF986_RS26315
• Protein ID: WP_004181778.1
• Coordinates: 45412..45696 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A5Q9LM53
• Locus tag: KF986_RS26310
• Protein ID: WP_025368601.1
• Coordinates: 45174..45422 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KF986_RS26290 (KF986_026290)	40384..41205	-	822	WP_004026535.1	hypothetical protein	-
KF986_RS26295 (KF986_026295)	41267..41620	-	354	WP_004181774.1	hypothetical protein	-
KF986_RS26300 (KF986_026300)	41765..42751	-	987	WP_025368599.1	hypothetical protein	-
KF986_RS26305 (KF986_026305)	43085..44884	-	1800	WP_088678991.1	ATP-dependent helicase	-
KF986_RS26310 (KF986_026310)	45174..45422	+	249	WP_025368601.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KF986_RS26315 (KF986_026315)	45412..45696	+	285	WP_004181778.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KF986_RS26320 (KF986_026320)	45926..47152	+	1227	WP_025368602.1	RNA-guided endonuclease TnpB family protein	-
KF986_RS26325 (KF986_026325)	47438..47863	-	426	WP_040120489.1	IS200/IS605 family transposase	-
KF986_RS26330 (KF986_026330)	47900..49126	+	1227	WP_025368604.1	RNA-guided endonuclease TnpB family protein	-
KF986_RS26335 (KF986_026335)	49192..50318	-	1127	Protein_66	conjugal transfer protein TraG N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaOXA-48 / catA1 / blaOXA-1 / aac(6')-Ib-cr / blaTEM-1B / qnrS1 / blaCTX-M-15 / ant(2'')-Ia / ant(3'')-Ia / qacE / sul1	iucA / iucB / iucC / iucD / iutA / rmpA2	1..326663	326663

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10970.69 Da
• Isoelectric Point: 10.6516

>T240906 WP_004181778.1 NZ_CP094992:45412-45696 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYNLTKHRN

Nucleotide

• Length: 285 bp

>T240906 NZ_LT853885:2004259-2004678 [Xanthomonas fragariae]
ATGATTGTCTTGGATACGAATGTAGTCTCCGAGGCGATAAAACCCGAACCCAACCCGGCCGTGCGAGCTTGGTTGAATGA
GCAGGTAGCGGAAACCCTCTACCTGTCCAGCGTGACGCTTGCCGAACTGCTGTTCGGCATCGGCGCGCTGCCGAACGGGA
AACGTAAGAAAGGCCTGGGCGAAGCCCTGGACGGCTTGCTCGAACTGTTCGGCGAGCGGGTGCTGATGTTCGACACCGAA
GCGGCTCGCCATTATGCCGAGCTGGCGGTGAAGGCCCGCACAGCCGGGAAGGGTTTTCCAACACCTGACGGATATATTGG
GGCTATTGCGGCCTCCAAGGGATTTATCGTAGCAACACGCGACACCAGCCCTTTCGAAGCGGCTGGGCTTACTGTTATCA
ATCCTTGGAACCATCAGTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8906.12 Da
• Isoelectric Point: 3.8206

>AT240906 WP_025368601.1 NZ_CP094992:45174-45422 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGHIADERIGEAVIEVSLD
DL

Nucleotide

• Length: 249 bp

>AT240906 NZ_LT853885:2004008-2004262 [Xanthomonas fragariae]
ATGGCAATCCTGACAGTGCGGAATGTGCCCGACGAGGTGCATCGCGCTCTGCGCTTACGGGCCGCCGAGCATGGCCGCAG
TACAGAGGCGGAGGTCCGCGAGATTCTGGAGAGTGCAGTTAAGTCAGAAAAGCGCATCCGCATGGGCGACGCGTTGGCGG
AGCTTGGCCGCCAAGTAGTGCTGACAAACGATGATTTCGCAGTGTTGGACCAGGTGCGCGACAAGGTGCCGGCAGAACCG
ATGAGGTTTGAATGA

Structures

Toxin

• AlphaFold DB: A0A2J4R0S6

Antitoxin

• AlphaFold DB: A0A5Q9LM53