Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5272401..5273026 Replicon chromosome
Accession NZ_CP094991
Organism Klebsiella pneumoniae strain SCPM-O-B-8919 (12PKP/19c)

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KF986_RS25640 Protein ID WP_002882817.1
Coordinates 5272401..5272784 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KF986_RS25645 Protein ID WP_004150355.1
Coordinates 5272784..5273026 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KF986_RS25625 (KF986_025625) 5269767..5270669 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KF986_RS25630 (KF986_025630) 5270666..5271301 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KF986_RS25635 (KF986_025635) 5271298..5272227 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KF986_RS25640 (KF986_025640) 5272401..5272784 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KF986_RS25645 (KF986_025645) 5272784..5273026 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KF986_RS25650 (KF986_025650) 5273231..5274148 + 918 WP_004187175.1 alpha/beta hydrolase -
KF986_RS25655 (KF986_025655) 5274162..5275103 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KF986_RS25660 (KF986_025660) 5275148..5275585 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KF986_RS25665 (KF986_025665) 5275582..5276442 - 861 WP_019725422.1 virulence factor BrkB family protein -
KF986_RS25670 (KF986_025670) 5276436..5277035 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T240905 WP_002882817.1 NZ_CP094991:c5272784-5272401 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T240905 NZ_LT853885:c302406-302254 [Xanthomonas fragariae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACTGGCTGTAACACCTTTGCTGGCGC
CGGCAAGGATATGCAACGCGCGGGCAGCAAGGTCGAAAAGAAGGCCGATAACTGCAGCGACGGTAAGTGCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT240905 WP_004150355.1 NZ_CP094991:c5273026-5272784 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT240905 NZ_LT853885:c302174-302040 [Xanthomonas fragariae]
ATGAAGCGTGCAATTGTGCTGCTGGTGCTGTCGGTGTTGTCGGTCGGTATACTGTCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGAAGGGCGCTGGCGAGAAGGTGGAAGACGCTGCGCGCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References