Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4667068..4667878 Replicon chromosome
Accession NZ_CP094991
Organism Klebsiella pneumoniae strain SCPM-O-B-8919 (12PKP/19c)

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag KF986_RS22820 Protein ID WP_002887280.1
Coordinates 4667068..4667601 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KF986_RS22825 Protein ID WP_002887278.1
Coordinates 4667612..4667878 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KF986_RS22815 (KF986_022815) 4665899..4667020 + 1122 WP_009309849.1 cupin domain-containing protein -
KF986_RS22820 (KF986_022820) 4667068..4667601 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
KF986_RS22825 (KF986_022825) 4667612..4667878 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KF986_RS22830 (KF986_022830) 4667981..4669414 - 1434 WP_019724905.1 Cu(+)/Ag(+) sensor histidine kinase -
KF986_RS22835 (KF986_022835) 4669404..4670087 - 684 WP_004214143.1 copper response regulator transcription factor CusR -
KF986_RS22840 (KF986_022840) 4670259..4671641 + 1383 WP_019724904.1 efflux transporter outer membrane subunit -
KF986_RS22845 (KF986_022845) 4671659..4672003 + 345 WP_025987660.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T240901 WP_002887280.1 NZ_CP094991:c4667601-4667068 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T240901 NZ_LT853883:c10294-10001 [Xanthomonas fragariae]
GTGCTCGTTCTTGAGTGGCGCGAGACAGCCCGCGCCGATCTGCTGGCGATTGTCGATTACATATCCGACGACAACCCGGA
TGCAGCGCAGCGGTTGAAAGATGACATCGAGGCCAAGGCCTCGATGCTGCCTGAGCGCCCGAAGCTGTATCGGCCTGGTC
GCGTCGCCGGCACTCGGGAAATGGTCGTCCGATCGAACTACGTGGTGGTGTACGCGGAAGACGCGCGCGCCGTTTCCATC
CTGCGGGTGCTGCATGCGGCCCAACAGTGGCCGCCAGTGACCGGGGTTGAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT240901 WP_002887278.1 NZ_CP094991:c4667878-4667612 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT240901 NZ_LT853883:c10580-10281 [Xanthomonas fragariae]
ATGGCTGCTCACACCTCGATGCTGCATATCCGCGTCGATGACGCTCTAAAGGCACAGGCTGCCGAGACGCTCGCCAATGT
CGGCCTGACGGTCTCGGATGCTGTGCGCATCCTGCTGACCCGCGTCGCCAAGGAAGGCGGGTTGCCAGCCGGCCTGACGG
CCGATCCAGAGGCCCACGACGCCTGGTTCCGGGGCAAGGTGCAAGAGGCCCTGGCCGACACGCGGCCGGCGGTGCCGCAT
CGCCAGGTGATGGATGAGGCGCAAGCCCTGATCGACCGGAAACGCCGTGCTCGTTCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References