Detailed information of TA system TA024090

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 3899917..3900731
• Replicon: chromosome
• Accession: NC_012947
• Organism: Escherichia coli BL21-Gold(DE3)pLysS AG

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: ECBD_RS19475
• Protein ID: WP_001054376.1
• Coordinates: 3899917..3900174 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: ECBD_RS19480
• Protein ID: WP_001309181.1
• Coordinates: 3900186..3900731 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECBD_RS19450	3895205..3896311	+	1107	WP_001309184.1	N-acetylneuraminate epimerase	-
ECBD_RS19455	3896376..3897356	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
ECBD_RS19460	3897939..3899179	-	1241	Protein_3709	helicase YjhR	-
ECBD_RS25735	3899295..3899540	+	246	Protein_3710	GNAT family N-acetyltransferase	-
ECBD_RS19475	3899917..3900174	+	258	WP_001054376.1	hypothetical protein	Toxin
ECBD_RS19480	3900186..3900731	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
ECBD_RS19485	3900787..3901533	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
ECBD_RS25255	3901702..3901920	+	219	Protein_3714	hypothetical protein	-
ECBD_RS25940	3901958..3902074	+	117	Protein_3715	VOC family protein	-
ECBD_RS19490	3902319..3903440	+	1122	WP_000010829.1	M42 family metallopeptidase	-
ECBD_RS19495	3903437..3903715	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
ECBD_RS19500	3903727..3905040	+	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	fimE / fimB	3890561..3942320	51759

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T24090 WP_001054376.1 NC_012947:3899917-3900174 [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T24090 NC_012947:3899917-3900174 [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT24090 WP_001309181.1 NC_012947:3900186-3900731 [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT24090 NC_012947:3900186-3900731 [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8