Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2673425..2675013 Replicon chromosome
Accession NZ_CP094991
Organism Klebsiella pneumoniae strain SCPM-O-B-8919 (12PKP/19c)

Toxin (Protein)


Gene name hipA Uniprot ID W8UI36
Locus tag KF986_RS13005 Protein ID WP_002904792.1
Coordinates 2673425..2674747 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A0H3GXD8
Locus tag KF986_RS13010 Protein ID WP_002904790.1
Coordinates 2674747..2675013 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KF986_RS12990 (KF986_012990) 2668481..2670982 + 2502 WP_064145444.1 fimbria/pilus outer membrane usher protein -
KF986_RS12995 (KF986_012995) 2670970..2671974 + 1005 WP_064145446.1 fimbrial protein -
KF986_RS13000 (KF986_013000) 2672116..2673408 + 1293 WP_064145447.1 glycoside hydrolase family 10 protein -
KF986_RS13005 (KF986_013005) 2673425..2674747 - 1323 WP_002904792.1 type II toxin-antitoxin system HipA family toxin Toxin
KF986_RS13010 (KF986_013010) 2674747..2675013 - 267 WP_002904790.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
KF986_RS13015 (KF986_013015) 2675401..2675727 - 327 WP_002904788.1 hydroxyisourate hydrolase -
KF986_RS13020 (KF986_013020) 2675724..2676224 - 501 WP_064145448.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
KF986_RS13025 (KF986_013025) 2676202..2677581 - 1380 WP_064145449.1 nucleobase:cation symporter-2 family protein -
KF986_RS13030 (KF986_013030) 2677927..2679081 + 1155 WP_023282572.1 FAD-dependent urate hydroxylase HpxO -
KF986_RS13035 (KF986_013035) 2679062..2679985 - 924 WP_064145450.1 LysR family hpxDE operon transcriptional regulator HpxR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - mgtC / iroE 2499025..2709971 210946


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(149-402)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-65)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49171.46 Da        Isoelectric Point: 7.5357

>T240899 WP_002904792.1 NZ_CP094991:c2674747-2673425 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Download         Length: 1323 bp

>T240899 NZ_LT853882:3991111-3991245 [Xanthomonas fragariae]
ATGAAGCGTGCAATTGTGCTGCTGGTGCTGTCGGTGTTGTCGGTCGGTATACTGTCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGAAGGGCGCTGGCGAGAAGGTGGAAGACGCTGCGCGCAAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9906.27 Da        Isoelectric Point: 8.5912

>AT240899 WP_002904790.1 NZ_CP094991:c2675013-2674747 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Download         Length: 267 bp

>AT240899 NZ_LT853882:3990879-3991031 [Xanthomonas fragariae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACTGGCTGTAACACCTTTGCTGGCGC
CGGCAAGGATATGCAACGCGCGGGCAGCAAGGTCGAAAAGAAGGCCGATAACTGCAGCGACGGTAAGTGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E1CEC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GXD8

References