Detailed information of TA system TA240883

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 11228..11775
• Replicon: plasmid pS3160_2
• Accession: NZ_CP094989
• Organism: Shigella flexneri strain 3160_NCHU22

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: MT485_RS24690
• Protein ID: WP_244442846.1
• Coordinates: 11497..11775 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: MT485_RS24685
• Protein ID: WP_000079941.1
• Coordinates: 11228..11497 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MT485_RS24655 (6404)	6404..6502	+	99	Protein_8	ANT(3'') family aminoglycoside nucleotidyltransferase	-
MT485_RS24660 (6669)	6669..7877	-	1209	WP_000198533.1	IS91 family transposase	-
MT485_RS24665 (7858)	7858..8130	-	273	WP_000019163.1	hypothetical protein	-
MT485_RS24670 (8345)	8345..9160	+	816	WP_001043260.1	sulfonamide-resistant dihydropteroate synthase Sul2	-
MT485_RS24675 (9247)	9247..9489	+	243	Protein_12	phosphoglucosamine mutase	-
MT485_RS24680 (9725)	9725..10870	-	1146	Protein_13	IS91-like element ISVsa3 family transposase	-
MT485_RS24685 (11228)	11228..11497	+	270	WP_000079941.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
MT485_RS24690 (11497)	11497..11775	+	279	WP_244442846.1	type II toxin-antitoxin system toxin YacB	Toxin
MT485_RS24695 (11815)	11815..12663	+	849	WP_021265674.1	3'-5' exonuclease	-
MT485_RS24700 (12780)	12780..13253	+	474	WP_001334658.1	hypothetical protein	-
MT485_RS24705 (13730)	13730..13996	-	267	WP_000955263.1	hypothetical protein	-
MT485_RS24710 (13996)	13996..14490	-	495	WP_001459505.1	hypothetical protein	-
MT485_RS24715 (14546)	14546..15148	-	603	WP_000517695.1	ProQ/FINO family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA12 / sul2	-	1..101193	101193

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10761.43 Da
• Isoelectric Point: 9.7617

>T240883 WP_244442846.1 NZ_CP094989:11497-11775 [Shigella flexneri]
MEIFWTMLASQDRKRIRKYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T240883 NZ_LT838200:3742907-3743185 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT240883 WP_000079941.1 NZ_CP094989:11228-11497 [Shigella flexneri]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT240883 NZ_LT838200:3742644-3742904 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A629TXC5