Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 101384..102141 Replicon plasmid pS3160_1
Accession NZ_CP094988
Organism Shigella flexneri strain 3160_NCHU22

Toxin (Protein)


Gene name tacT Uniprot ID D2AJG5
Locus tag MT485_RS23910 Protein ID WP_000501974.1
Coordinates 101384..101869 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q31SN8
Locus tag MT485_RS23915 Protein ID WP_011114751.1
Coordinates 101857..102141 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MT485_RS23880 (MT485_23880) 96908..97332 - 425 Protein_118 hypothetical protein -
MT485_RS23885 (MT485_23885) 97560..98198 - 639 WP_000502854.1 ParB N-terminal domain-containing protein -
MT485_RS23890 (MT485_23890) 98183..98326 - 144 Protein_120 DUF3440 domain-containing protein -
MT485_RS23905 (MT485_23905) 100930..101211 + 282 Protein_123 IS4/IS5 family transposase -
MT485_RS23910 (MT485_23910) 101384..101869 - 486 WP_000501974.1 GNAT family N-acetyltransferase Toxin
MT485_RS23915 (MT485_23915) 101857..102141 - 285 WP_011114751.1 DUF1778 domain-containing protein Antitoxin
MT485_RS23920 (MT485_23920) 103043..103207 + 165 WP_001346193.1 hypothetical protein -
MT485_RS23925 (MT485_23925) 103461..105062 - 1602 WP_005067462.1 IS66-like element ISSfl3 family transposase -
MT485_RS23930 (MT485_23930) 105059..105409 - 351 WP_005048975.1 IS66 family insertion sequence element accessory protein TnpB -
MT485_RS23935 (MT485_23935) 105406..106080 - 675 WP_004967157.1 IS66-like element accessory protein TnpA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ipaH1.4 / ospE1 / nleE / icsP/sopA / ospB / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ospE1 / ipaH2.5 / ospC2 / ipaH7.8 / ipaH4.5 / ospD3/senA / ospC1 / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG 1..222049 222049
- inside IScluster/Tn - ipaH7.8 / ipaH4.5 / ospD3/senA / ospC1 / ospC3 73168..114071 40903


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(27-151)

Antitoxin

Vibrio phage ICP1, Orf50

(12-88)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17814.63 Da        Isoelectric Point: 9.8822

>T240879 WP_000501974.1 NZ_CP094988:c101869-101384 [Shigella flexneri]
MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATRTFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
Q

Download         Length: 486 bp

>T240879 NZ_LT838200:2739320-2739427 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10871.52 Da        Isoelectric Point: 4.6812

>AT240879 WP_011114751.1 NZ_CP094988:c102141-101857 [Shigella flexneri]
MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDP
AIEKLLARKPQWDV

Download         Length: 285 bp

>AT240879 NZ_LT838200:c2739273-2739207 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PN28

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CUJ1

References