Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3859283..3860084 Replicon chromosome
Accession NZ_CP094987
Organism Shigella flexneri strain 3160_NCHU22

Toxin (Protein)


Gene name yeeV Uniprot ID D3GUT5
Locus tag MT485_RS19475 Protein ID WP_001094430.1
Coordinates 3859707..3860084 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID D3GUT4
Locus tag MT485_RS19470 Protein ID WP_001285620.1
Coordinates 3859283..3859660 (+) Length 126 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MT485_RS19440 (3855904) 3855904..3856359 + 456 WP_005051844.1 IrmA family protein -
MT485_RS19445 (3856502) 3856502..3856591 + 90 WP_112031555.1 DUF905 family protein -
MT485_RS19450 (3856770) 3856770..3857588 + 819 WP_001177758.1 DUF932 domain-containing protein -
MT485_RS19455 (3857930) 3857930..3858403 + 474 WP_000855059.1 antirestriction protein -
MT485_RS19460 (3858419) 3858419..3858895 + 477 WP_001387238.1 RadC family protein -
MT485_RS19465 (3858982) 3858982..3859203 + 222 WP_000692350.1 DUF987 domain-containing protein -
MT485_RS19470 (3859283) 3859283..3859660 + 378 WP_001285620.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
MT485_RS19475 (3859707) 3859707..3860084 + 378 WP_001094430.1 TA system toxin CbtA family protein Toxin
MT485_RS19480 (3860081) 3860081..3860569 + 489 WP_000761715.1 DUF5983 family protein -
MT485_RS19485 (3860589) 3860589..3860786 + 198 WP_000772029.1 DUF957 domain-containing protein -
MT485_RS19490 (3860871) 3860871..3861713 + 843 WP_001290187.1 DUF4942 domain-containing protein -
MT485_RS19495 (3862490) 3862490..3862735 + 246 WP_000875412.1 hypothetical protein -
MT485_RS19500 (3863138) 3863138..3864300 + 1163 WP_094081514.1 IS3-like element IS3 family transposase -
MT485_RS19505 (3864404) 3864404..3864871 - 468 WP_011069513.1 prepilin peptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13971.82 Da        Isoelectric Point: 6.8608

>T240875 WP_001094430.1 NZ_CP094987:3859707-3860084 [Shigella flexneri]
MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ

Download         Length: 378 bp

>T240875 NZ_LT838200:2009330-2009433 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 13698.52 Da        Isoelectric Point: 5.9505

>AT240875 WP_001285620.1 NZ_CP094987:3859283-3859660 [Shigella flexneri]
VSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPATTV

Download         Length: 378 bp

>AT240875 NZ_LT838200:c2009471-2009200 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y6M7

Antitoxin

Source ID Structure
PDB 2INW
AlphaFold DB A0A0E0Y522

References