Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3736170..3736824 Replicon chromosome
Accession NZ_CP094987
Organism Shigella flexneri strain 3160_NCHU22

Toxin (Protein)


Gene name cptA Uniprot ID Q0T101
Locus tag MT485_RS18865 Protein ID WP_000244767.1
Coordinates 3736170..3736577 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID D2A730
Locus tag MT485_RS18870 Protein ID WP_000354052.1
Coordinates 3736558..3736824 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MT485_RS18845 (3732127) 3732127..3733860 - 1734 WP_000813187.1 single-stranded-DNA-specific exonuclease RecJ -
MT485_RS18850 (3733866) 3733866..3734576 - 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
MT485_RS18855 (3734601) 3734601..3735497 - 897 WP_000806987.1 site-specific tyrosine recombinase XerD -
MT485_RS18860 (3735609) 3735609..3736130 + 522 WP_001055867.1 flavodoxin FldB -
MT485_RS18865 (3736170) 3736170..3736577 - 408 WP_000244767.1 protein YgfX Toxin
MT485_RS18870 (3736558) 3736558..3736824 - 267 WP_000354052.1 FAD assembly factor SdhE Antitoxin
MT485_RS18875 (3737067) 3737067..3738047 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
MT485_RS18880 (3738243) 3738243..3738902 - 660 WP_000250274.1 hemolysin III family protein -
MT485_RS18885 (3739066) 3739066..3739376 - 311 Protein_3667 N(4)-acetylcytidine aminohydrolase -
MT485_RS18890 (3739421) 3739421..3740854 + 1434 WP_005051722.1 6-phospho-beta-glucosidase BglA -
MT485_RS18895 (3740911) 3740911..3741654 - 744 WP_000951957.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15978.91 Da        Isoelectric Point: 10.9373

>T240874 WP_000244767.1 NZ_CP094987:c3736577-3736170 [Shigella flexneri]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T240874 NZ_LT838200:c1848286-1847921 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10519.05 Da        Isoelectric Point: 5.1599

>AT240874 WP_000354052.1 NZ_CP094987:c3736824-3736558 [Shigella flexneri]
MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT240874 NZ_LT838200:c1848744-1848376 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TIU0

Antitoxin

Source ID Structure
AlphaFold DB D2A730

References