Detailed information of TA system TA024087

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3368072..3368909
• Replicon: chromosome
• Accession: NC_012947
• Organism: Escherichia coli BL21-Gold(DE3)pLysS AG

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: A0A140NB16
• Locus tag: ECBD_RS16885
• Protein ID: WP_000227786.1
• Coordinates: 3368367..3368909 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: ECBD_RS16880
• Protein ID: WP_001297137.1
• Coordinates: 3368072..3368383 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECBD_RS16855	3363092..3364039	+	948	WP_001239440.1	cytochrome o ubiquinol oxidase subunit II	-
ECBD_RS16860	3364061..3366052	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
ECBD_RS16865	3366042..3366656	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
ECBD_RS16870	3366656..3366985	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
ECBD_RS16875	3366997..3367887	+	891	WP_000971336.1	heme o synthase	-
ECBD_RS16880	3368072..3368383	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
ECBD_RS16885	3368367..3368909	+	543	WP_000227786.1	GNAT family N-acetyltransferase	Toxin
ECBD_RS16890	3368965..3369900	-	936	WP_001297127.1	sel1 repeat family protein	-
ECBD_RS16895	3370317..3371681	+	1365	WP_001000978.1	MFS transporter	-
ECBD_RS16900	3371809..3372300	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
ECBD_RS16905	3372468..3373379	+	912	WP_000705853.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19794.98 Da
• Isoelectric Point: 8.3395

>T24087 WP_000227786.1 NC_012947:3368367-3368909 [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADSAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T24087 NC_012947:3368367-3368909 [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACTCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT24087 WP_001297137.1 NC_012947:3368072-3368383 [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT24087 NC_012947:3368072-3368383 [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A140NB16

Antitoxin

• AlphaFold DB: A0A0E0Y6B3