Detailed information of TA system TA240863

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2343686..2344212
• Replicon: chromosome
• Accession: NZ_CP094987
• Organism: Shigella flexneri strain 3160_NCHU22

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: MT485_RS11820
• Protein ID: WP_000323025.1
• Coordinates: 2343686..2343973 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: MT485_RS11825
• Protein ID: WP_000534858.1
• Coordinates: 2343973..2344212 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MT485_RS11770 (2338744)	2338744..2339079	+	336	WP_000871291.1	anti-adapter protein IraM	-
MT485_RS11775 (2339360)	2339360..2339468	-	109	Protein_2285	DUF3927 family protein	-
MT485_RS11790 (2340389)	2340389..2341210	-	822	WP_000762882.1	antitermination protein	-
MT485_RS11795 (2341225)	2341225..2341581	-	357	WP_000904111.1	RusA family crossover junction endodeoxyribonuclease	-
MT485_RS11800 (2341594)	2341594..2342643	-	1050	WP_001265249.1	DUF968 domain-containing protein	-
MT485_RS11805 (2342645)	2342645..2342923	-	279	Protein_2289	hypothetical protein	-
MT485_RS11810 (2342990)	2342990..2343241	-	252	WP_000980987.1	protein Rem	-
MT485_RS11815 (2343459)	2343459..2343614	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
MT485_RS11820 (2343686)	2343686..2343973	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
MT485_RS11825 (2343973)	2343973..2344212	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
MT485_RS11830 (2344237)	2344237..2344542	+	306	WP_071818640.1	hypothetical protein	-
MT485_RS11835 (2344539)	2344539..2345695	-	1157	Protein_2295	IS3-like element IS600 family transposase	-
MT485_RS11840 (2345757)	2345757..2346776	+	1020	Protein_2296	MHS family MFS transporter YdfJ	-
MT485_RS11845 (2346865)	2346865..2348325	+	1461	WP_000527804.1	mannitol dehydrogenase family protein	-
MT485_RS11850 (2348361)	2348361..2348564	-	204	WP_000214712.1	putative selenium delivery protein YdfZ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2327657..2349428	21771

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T240863 WP_000323025.1 NZ_CP094987:c2343973-2343686 [Shigella flexneri]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T240863 NZ_LT838196:c4341298-4341125 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT240863 WP_000534858.1 NZ_CP094987:c2344212-2343973 [Shigella flexneri]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT240863 NZ_LT838196:c4341622-4341326 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8