Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3332630..3333248 Replicon chromosome
Accession NC_012947
Organism Escherichia coli BL21-Gold(DE3)pLysS AG

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag ECBD_RS16710 Protein ID WP_001291435.1
Coordinates 3333030..3333248 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag ECBD_RS16705 Protein ID WP_000344800.1
Coordinates 3332630..3333004 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECBD_RS16695 3327719..3328912 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
ECBD_RS16700 3328935..3332084 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
ECBD_RS16705 3332630..3333004 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
ECBD_RS16710 3333030..3333248 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
ECBD_RS16715 3333420..3333971 + 552 WP_000102564.1 maltose O-acetyltransferase -
ECBD_RS16720 3334087..3334557 + 471 WP_000136192.1 YlaC family protein -
ECBD_RS16725 3334721..3336271 + 1551 WP_001319841.1 cyclic-guanylate-specific phosphodiesterase PdeB -
ECBD_RS16730 3336313..3336666 - 354 WP_000878140.1 DUF1428 domain-containing protein -
ECBD_RS16735 3337045..3337356 + 312 WP_000409911.1 MGMT family protein -
ECBD_RS16740 3337387..3337959 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T24086 WP_001291435.1 NC_012947:3333030-3333248 [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T24086 NC_012947:3333030-3333248 [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT24086 WP_000344800.1 NC_012947:3332630-3333004 [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT24086 NC_012947:3332630-3333004 [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References