Detailed information of TA system TA240853

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 3156672..3157258
• Replicon: chromosome
• Accession: NZ_CP094975
• Organism: Pseudomonas sp. PIA16

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: L9B60_RS14325
• Protein ID: WP_249679457.1
• Coordinates: 3156672..3156971 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: L9B60_RS14330
• Protein ID: WP_249679458.1
• Coordinates: 3156968..3157258 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L9B60_RS14300	3151984..3152445	-	462	WP_249679743.1	Lrp/AsnC family transcriptional regulator	-
L9B60_RS14305	3152511..3152618	-	108	Protein_2815	transcriptional regulator	-
L9B60_RS14310	3152677..3154584	-	1908	WP_249679454.1	acyltransferase family protein	-
L9B60_RS14315	3154869..3155822	+	954	WP_249679455.1	NAD-dependent epimerase/dehydratase family protein	-
L9B60_RS14320	3156318..3156551	+	234	WP_249679456.1	hypothetical protein	-
L9B60_RS14325	3156672..3156971	+	300	WP_249679457.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L9B60_RS14330	3156968..3157258	+	291	WP_249679458.1	putative addiction module antidote protein	Antitoxin
L9B60_RS14335	3157726..3158904	-	1179	WP_249679459.1	P-type conjugative transfer protein TrbL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11033.70 Da
• Isoelectric Point: 10.4514

>T240853 WP_249679457.1 NZ_CP094975:3156672-3156971 [Pseudomonas sp. PIA16]
MIGIKQTATFMTWESKLKDQRAKAAIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIETAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T240853 NZ_LT838196:2295716-2296003 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10280.79 Da
• Isoelectric Point: 4.7483

>AT240853 WP_249679458.1 NZ_CP094975:3156968-3157258 [Pseudomonas sp. PIA16]
MTEPIYDYDPAQALDGPEAIAVFIADALDTGDAAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSEHGNPTLKTMLV
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT240853 NZ_LT838196:2295477-2295716 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA