Detailed information of TA system TA240850

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 381760..382343
• Replicon: chromosome
• Accession: NZ_CP094975
• Organism: Pseudomonas sp. PIA16

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: L9B60_RS01665
• Protein ID: WP_249679636.1
• Coordinates: 381760..382014 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: L9B60_RS01670
• Protein ID: WP_249675528.1
• Coordinates: 382011..382343 (+)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L9B60_RS01650	377176..378378	+	1203	WP_249675521.1	multidrug effflux MFS transporter	-
L9B60_RS01655	378410..379174	+	765	WP_249675523.1	SDR family NAD(P)-dependent oxidoreductase	-
L9B60_RS01660	379213..381603	+	2391	WP_249675525.1	membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family	-
L9B60_RS01665	381760..382014	+	255	WP_249679636.1	type II toxin-antitoxin system HicA family toxin	Toxin
L9B60_RS01670	382011..382343	+	333	WP_249675528.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
L9B60_RS01675	382554..382916	-	363	WP_249675531.1	hypothetical protein	-
L9B60_RS01680	383349..384248	-	900	WP_249675533.1	LysR family transcriptional regulator	-
L9B60_RS01685	385000..386121	-	1122	WP_249675536.1	hypothetical protein	-
L9B60_RS01690	386156..386776	-	621	WP_249675537.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-82)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9370.62 Da
• Isoelectric Point: 10.2710

>T240850 WP_249679636.1 NZ_CP094975:381760-382014 [Pseudomonas sp. PIA16]
INSKQTSTLKSIFEKPVPNTLEWARIESLFMAVGAKTTEGNGSRVRFELNGVVGTFHRPHPDKEAKPYQVRDARSFLEQA
GVTP

Nucleotide

• Length: 255 bp

>T240850 NZ_LT838196:1832755-1833009 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12186.68 Da
• Isoelectric Point: 5.9732

>AT240850 WP_249675528.1 NZ_CP094975:382011-382343 [Pseudomonas sp. PIA16]
MSAMNYKGYAARIEYSEEDGLFVGHIAGIRDVVGFHGESVQELRTAFYEAVDDYLSACEKFGRAPQKPYSGKLSLRLEPE
LHATVALKAELSHKSINQWVSDALNQAVHG

Nucleotide

• Length: 333 bp

>AT240850 NZ_LT838196:1832507-1832758 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA