Detailed information of TA system TA024085

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2318396..2319034
• Replicon: chromosome
• Accession: NC_012947
• Organism: Escherichia coli BL21-Gold(DE3)pLysS AG

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: ECBD_RS11420
• Protein ID: WP_000813794.1
• Coordinates: 2318858..2319034 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: ECBD_RS11415
• Protein ID: WP_001270286.1
• Coordinates: 2318396..2318812 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECBD_RS11395	2313548..2314489	-	942	WP_001251313.1	ABC transporter permease	-
ECBD_RS11400	2314490..2315503	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
ECBD_RS11405	2315521..2316666	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
ECBD_RS11410	2316911..2318317	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
ECBD_RS11415	2318396..2318812	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
ECBD_RS11420	2318858..2319034	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
ECBD_RS11425	2319256..2319486	+	231	WP_000494244.1	YncJ family protein	-
ECBD_RS11430	2319578..2321539	-	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
ECBD_RS11435	2321612..2322148	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
ECBD_RS11440	2322201..2323415	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T24085 WP_000813794.1 NC_012947:c2319034-2318858 [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T24085 NC_012947:c2319034-2318858 [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT24085 WP_001270286.1 NC_012947:c2318812-2318396 [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT24085 NC_012947:c2318812-2318396 [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB