Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2652580..2653231 Replicon chromosome
Accession NZ_CP094959
Organism Lacticaseibacillus paracasei strain VHProbi M56

Toxin (Protein)


Gene name pemK Uniprot ID K6S8L5
Locus tag MUB27_RS12875 Protein ID WP_003567661.1
Coordinates 2652580..2652963 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A829TRS3
Locus tag MUB27_RS12880 Protein ID WP_003606045.1
Coordinates 2652983..2653231 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUB27_RS12870 (MUB27_12845) 2651875..2652396 - 522 WP_003567657.1 QueT transporter family protein -
MUB27_RS12875 (MUB27_12850) 2652580..2652963 - 384 WP_003567661.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MUB27_RS12880 (MUB27_12855) 2652983..2653231 - 249 WP_003606045.1 programmed cell death antitoxin Antitoxin
MUB27_RS12885 (MUB27_12860) 2653299..2654435 - 1137 WP_003606047.1 alanine racemase -
MUB27_RS12890 (MUB27_12865) 2654422..2654796 - 375 WP_003567669.1 holo-ACP synthase -
MUB27_RS12895 (MUB27_12870) 2654966..2656474 - 1509 WP_003567670.1 DEAD/DEAH box helicase -
MUB27_RS12900 (MUB27_12875) 2656774..2658162 - 1389 WP_003606050.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13773.03 Da        Isoelectric Point: 10.9764

>T240849 WP_003567661.1 NZ_CP094959:c2652963-2652580 [Lacticaseibacillus paracasei]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T240849 NZ_LT838196:1832507-1832758 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8968.11 Da        Isoelectric Point: 4.4610

>AT240849 WP_003606045.1 NZ_CP094959:c2653231-2652983 [Lacticaseibacillus paracasei]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQTVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT240849 NZ_LT838196:1832755-1833009 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K6S8L5

Antitoxin

Source ID Structure
AlphaFold DB A0A829TRS3

References