Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2686034..2686685 Replicon chromosome
Accession NZ_CP094946
Organism Lacticaseibacillus paracasei strain VHProbi F11

Toxin (Protein)


Gene name pemK Uniprot ID K6S8L5
Locus tag MUB19_RS13120 Protein ID WP_003567661.1
Coordinates 2686034..2686417 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K6RKI5
Locus tag MUB19_RS13125 Protein ID WP_003567665.1
Coordinates 2686437..2686685 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUB19_RS13115 (MUB19_13070) 2685329..2685850 - 522 WP_003567657.1 QueT transporter family protein -
MUB19_RS13120 (MUB19_13075) 2686034..2686417 - 384 WP_003567661.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MUB19_RS13125 (MUB19_13080) 2686437..2686685 - 249 WP_003567665.1 antitoxin Antitoxin
MUB19_RS13130 (MUB19_13085) 2686753..2687889 - 1137 WP_003581082.1 alanine racemase -
MUB19_RS13135 (MUB19_13090) 2687876..2688250 - 375 WP_003567669.1 holo-ACP synthase -
MUB19_RS13140 (MUB19_13095) 2688419..2689927 - 1509 WP_003567670.1 DEAD/DEAH box helicase -
MUB19_RS13145 (MUB19_13100) 2690053..2690196 - 144 WP_016380391.1 hypothetical protein -
MUB19_RS13150 (MUB19_13105) 2690227..2691615 - 1389 WP_003581096.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13773.03 Da        Isoelectric Point: 10.9764

>T240847 WP_003567661.1 NZ_CP094946:c2686417-2686034 [Lacticaseibacillus paracasei]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T240847 NZ_LT838196:866799-867206 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8938.08 Da        Isoelectric Point: 4.4610

>AT240847 WP_003567665.1 NZ_CP094946:c2686685-2686437 [Lacticaseibacillus paracasei]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT240847 NZ_LT838196:866552-866818 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K6S8L5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BNY2

References