Detailed information of TA system TA240827

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 17417..18087
• Replicon: plasmid unnamed1
• Accession: NZ_CP094941
• Organism: Klebsiella pneumoniae strain FK1979

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: MUB43_RS25825
• Protein ID: WP_004213072.1
• Coordinates: 17644..18087 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: MUB43_RS25820
• Protein ID: WP_004213073.1
• Coordinates: 17417..17647 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUB43_RS25795 (MUB43_25795)	13640..14539	-	900	WP_004225022.1	nucleotide-binding protein	-
MUB43_RS25800 (MUB43_25800)	14529..14819	-	291	WP_004213078.1	hypothetical protein	-
MUB43_RS25805 (MUB43_25805)	15171..15377	+	207	WP_004213077.1	hypothetical protein	-
MUB43_RS25810 (MUB43_25810)	15367..15660	-	294	WP_004213076.1	hypothetical protein	-
MUB43_RS25815 (MUB43_25815)	15676..16809	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
MUB43_RS25820 (MUB43_25820)	17417..17647	+	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MUB43_RS25825 (MUB43_25825)	17644..18087	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
MUB43_RS25830 (MUB43_25830)	18236..18487	+	252	WP_186987481.1	hypothetical protein	-
MUB43_RS25835 (MUB43_25835)	18510..18814	-	305	Protein_21	transposase	-
MUB43_RS25840 (MUB43_25840)	19231..19869	+	639	Protein_22	mucoid phenotype regulator RmpA2	-
MUB43_RS25845 (MUB43_25845)	20387..20790	-	404	Protein_23	GAF domain-containing protein	-
MUB43_RS25850 (MUB43_25850)	20881..21801	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
MUB43_RS25855 (MUB43_25855)	21850..22341	-	492	WP_011251327.1	DM13 domain-containing protein	-
MUB43_RS25860 (MUB43_25860)	22404..22679	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA2 / rmpA / iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroN / rmpA	1..227097	227097

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T240827 WP_004213072.1 NZ_CP094941:17644-18087 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T240827 NZ_LT827011:c1155818-1155162 [Escherichia coli O127:H6]
ATGGCCTATGACTACAGCGCCAGCCTCAACCCGCAAAAAGCCCTGATCTGGCGTATCGTCCATCGGGACAATATCCCGTG
GATTCTGGATAACGGGCTGCACTGCGGCAACAGTCTGGTGCAGGCGGAAAACTGGATCAATATCGGCAACCCGGAGCTGA
TCGGCAAGCGTGCCGGGCATCCGGTACCTGTCGGAACGGGCGGGACACTGCATGACTATGTGCCGTTTTATTTTACCCCC
TTCTCGCCAATGCTGATGAACATCCACAGCGGGCGCGGCGGCATTAAGCGACGCCCCAATGAGGAGATCGTTATTCTGGT
CAGCAACTTGCGGAACGTGGCAGCACACGACGTGCCCTTCGTGTTTACCGACAGCCATGCTTACTATAACTGGACCAACT
ACTACACCAGTCTGAATAGTCTGGACCAGATCGACTGGCCCATTTTGCAGGCACGGGACTTTCGCCGCGATCCAGACGAT
CCGGCTAAATTTGAACGCTATCAGGCAGAGGCGCTAATCTGGCAACACTGCCCGATCTCTCTGCTGGATGGGATAATTTG
TTACAGCGAAGAGGTCAGGTTACAATTAGAACAATGGCTGTTTCAGCGTAATCTAACAATGTCCGTTCACACACGGTCTG
GATGGTACTTCTCATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT240827 WP_004213073.1 NZ_CP094941:17417-17647 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT240827 NZ_LT827011:c1155165-1154098 [Escherichia coli O127:H6]
ATGATCACATATACACAAGGCAATTTACTGGACGCACCGGTGGAAGCTCTGGTGAATACGGTTAATACCGTCGGTGTCAT
GGGGAAAGGCATCGCGTTGATGTTTAAAGAGCGCTTCCCCGAAAATATGAAAGTCTATGCCCTCGCCTGCAAACAAAAGC
AGGTGATCACCGGCAAAATGTTCATTACCGAAACCGGGGAACTGATGGGACCGCGCTGGATTGTTAATTTTCCGACCAAA
CAGCACTGGCGCGCTGATTCCCGCATGGAGTGGATTGAAGATGGCTTGCAGGACTTACGCCGCTTTTTGATTGAAGAGAA
CGTGCAGTCCATCGCCATTCCACCACTGGGCGCAGGCAACGGTGGTTTGAACTGGCCGGACGTTCGGGCGCAGATTGAAT
CTGCCCTTGGCGACCTTCAGGACGTTGATATCCTGATTTACCAACCGACTGAAAAATACCAGAACGTTGCCAAAAGCACT
GGCGTAAAAAAGCTCACTCCCGCCAGAGCCGCCATCGCTGAACTGGTGCGTCGCTACTGGGTATTGGGGATGGAGTGCAG
CCTACTGGAAATCCAGAAACTGGCCTGGCTGTTACAACGCGCCATTGAGCAGCATCAACAGGACGATATCCTCAAACTGC
GGTTTGAAGCTCACTATTATGGCCCCTATGCGCCCAATCTGAACCATCTGCTTAATGCGCTGGACGGCACCTATCTTAAA
GCCGAGAAACGCATCCCGGACAGCCAACCGCTGGATGTGATCTGGTTTAACGATCAGAAAAAAGAACACGTTAATGCGTA
TCTGAACAACGAAGCCAGAGAGTGGCTACCTGCGCTGGAGCAGGTCAGCCAGTTAATTGACGGCTTTGAATCGCCGTTTG
GTCTGGAGTTGCTGGCAACCGTAGACTGGTTGCTGAGCCGTGGCGAATGTCAACCAACGCTGGACTCCGTTAAGGAAGGG
CTGCACCAGTGGCCTGCGGGCGAACGCTGGGCCAGCCGTAAGTTGAGGCTGTTTGATAATAATAATCTGCAATTTGCCAT
TAACCGCGTAATGGAGTTCCATTGCTGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620