240826

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5244173..5244798 Replicon chromosome
Accession NZ_CP094940
Organism Klebsiella pneumoniae strain FK1979

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag MUB43_RS25360 Protein ID WP_002882817.1
Coordinates 5244173..5244556 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MUB43_RS25365 Protein ID WP_004150355.1
Coordinates 5244556..5244798 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUB43_RS25345 (5241539) 5241539..5242441 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
MUB43_RS25350 (5242438) 5242438..5243073 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
MUB43_RS25355 (5243070) 5243070..5243999 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
MUB43_RS25360 (5244173) 5244173..5244556 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
MUB43_RS25365 (5244556) 5244556..5244798 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MUB43_RS25370 (5245003) 5245003..5245920 + 918 WP_004213381.1 alpha/beta hydrolase -
MUB43_RS25375 (5245935) 5245935..5246876 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
MUB43_RS25380 (5246921) 5246921..5247358 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
MUB43_RS25385 (5247355) 5247355..5248215 - 861 WP_004146232.1 virulence factor BrkB family protein -
MUB43_RS25390 (5248209) 5248209..5248808 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T240826 WP_002882817.1 NZ_CP094940:c5244556-5244173 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T240826 NZ_LT827011:c440786-440568 [Escherichia coli O127:H6]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT240826 WP_004150355.1 NZ_CP094940:c5244798-5244556 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT240826 NZ_LT827011:c441186-440812 [Escherichia coli O127:H6]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References