Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4650797..4651607 Replicon chromosome
Accession NZ_CP094940
Organism Klebsiella pneumoniae strain FK1979

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag MUB43_RS22580 Protein ID WP_004178461.1
Coordinates 4650797..4651330 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag MUB43_RS22585 Protein ID WP_002887278.1
Coordinates 4651341..4651607 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUB43_RS22575 (4649628) 4649628..4650749 + 1122 WP_004214138.1 cupin domain-containing protein -
MUB43_RS22580 (4650797) 4650797..4651330 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
MUB43_RS22585 (4651341) 4651341..4651607 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
MUB43_RS22590 (4651710) 4651710..4653143 - 1434 WP_004214141.1 Cu(+)/Ag(+) sensor histidine kinase -
MUB43_RS22595 (4653133) 4653133..4653816 - 684 WP_004214143.1 copper response regulator transcription factor CusR -
MUB43_RS22600 (4653988) 4653988..4655373 + 1386 WP_004214145.1 efflux transporter outer membrane subunit -
MUB43_RS22605 (4655391) 4655391..4655735 + 345 WP_004214146.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T240822 WP_004178461.1 NZ_CP094940:c4651330-4650797 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T240822 NZ_LT795507:c17024-16773 [Escherichia coli]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATCACATTGTATGATGCCGTGCTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT240822 WP_002887278.1 NZ_CP094940:c4651607-4651341 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT240822 NZ_LT795507:c16786-16559 [Escherichia coli]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References