Detailed information of TA system TA240820

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2696823..2698411
• Replicon: chromosome
• Accession: NZ_CP094940
• Organism: Klebsiella pneumoniae strain FK1979

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: MUB43_RS13185
• Protein ID: WP_004213069.1
• Coordinates: 2696823..2698145 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: MUB43_RS13190
• Protein ID: WP_004176198.1
• Coordinates: 2698145..2698411 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUB43_RS13170 (2691879)	2691879..2694380	+	2502	WP_004213067.1	fimbria/pilus outer membrane usher protein	-
MUB43_RS13175 (2694368)	2694368..2695372	+	1005	WP_004213068.1	fimbrial protein	-
MUB43_RS13180 (2695514)	2695514..2696806	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
MUB43_RS13185 (2696823)	2696823..2698145	-	1323	WP_004213069.1	type II toxin-antitoxin system HipA family toxin	Toxin
MUB43_RS13190 (2698145)	2698145..2698411	-	267	WP_004176198.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
MUB43_RS13195 (2698676)	2698676..2699002	-	327	WP_004215084.1	hydroxyisourate hydrolase	-
MUB43_RS13200 (2698999)	2698999..2699499	-	501	WP_004215086.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
MUB43_RS13205 (2699477)	2699477..2700856	-	1380	WP_004215088.1	nucleobase:cation symporter-2 family protein	-
MUB43_RS13210 (2701202)	2701202..2702356	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
MUB43_RS13215 (2702337)	2702337..2703260	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	mgtC / iroE	2505680..2733244	227564

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49128.44 Da
• Isoelectric Point: 7.5357

>T240820 WP_004213069.1 NZ_CP094940:c2698145-2696823 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQATGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T240820 NZ_LT795506:21635-21895 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCCGATCTTGTTTTTCATGAGAAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.36 Da
• Isoelectric Point: 8.5912

>AT240820 WP_004176198.1 NZ_CP094940:c2698411-2698145 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT240820 NZ_LT795506:21885-22136 [Escherichia coli]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTCAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA