240808

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 199238..199868 Replicon chromosome
Accession NZ_CP094933
Organism Paraclostridium bifermentans strain HD0315_2

Toxin (Protein)


Gene name mazF Uniprot ID T4VJE6
Locus tag MTR78_RS01250 Protein ID WP_021430410.1
Coordinates 199503..199868 (+) Length 122 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A0M3DH37
Locus tag MTR78_RS01245 Protein ID WP_035118413.1
Coordinates 199238..199513 (+) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MTR78_RS01215 (MTR78_01215) 194680..196074 + 1395 WP_021430421.1 F0F1 ATP synthase subunit beta -
MTR78_RS01220 (MTR78_01220) 196077..196337 + 261 WP_021430426.1 ATP synthase F1 subunit epsilon -
MTR78_RS01225 (MTR78_01225) 196558..196938 + 381 WP_247012481.1 holo-ACP synthase -
MTR78_RS01230 (MTR78_01230) 196943..197404 + 462 WP_021434186.1 CBS domain-containing protein -
MTR78_RS01235 (MTR78_01235) 197427..198017 + 591 WP_247012483.1 germination lipoprotein GerS -
MTR78_RS01240 (MTR78_01240) 198032..199195 + 1164 WP_148552003.1 alanine racemase -
MTR78_RS01245 (MTR78_01245) 199238..199513 + 276 WP_035118413.1 hypothetical protein Antitoxin
MTR78_RS01250 (MTR78_01250) 199503..199868 + 366 WP_021430410.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MTR78_RS01255 (MTR78_01255) 200033..200860 + 828 WP_247012485.1 transketolase -
MTR78_RS01260 (MTR78_01260) 200853..201788 + 936 WP_025161276.1 transketolase family protein -
MTR78_RS01265 (MTR78_01265) 201907..202332 + 426 WP_025161277.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-118)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 13480.63 Da        Isoelectric Point: 8.0154

>T240808 WP_021430410.1 NZ_CP094933:199503-199868 [Paraclostridium bifermentans]
VSSNNLDIKRGHLFYADLSPVVGSEQGGIRPVLIIQNDIGNKYSPTVIVAAITSQINKAKLPTHIEISSNEYGLNKDSVI
LLEQIRTIDKKRLREKIGYLDECMMEKVNNSLLISLGLFQI

Download         Length: 366 bp

>T240808 NZ_LT795502:2428278-2429600 [Escherichia coli]
ATGCCCAAACTAGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACTTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCGTCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCTCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGGGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGAATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGGTATG
ATTTTATGAAATTCCAGGTCTTTCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTATTGGGTGGCGCGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGTACTGGATAACGTGAAGACTTCATTACCAACAGATTTTCCAGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACGGTCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 10338.72 Da        Isoelectric Point: 4.3885

>AT240808 WP_035118413.1 NZ_CP094933:199238-199513 [Paraclostridium bifermentans]
MSKEKIVFTLPDSLISEVDHIVQIENSNREDFAKAAFQFYITQKKKFDLKESMIKGYKEMGQINLTLAELGMTNEVSSDD
NLEGTIAQGEF

Download         Length: 276 bp

>AT240808 NZ_LT795502:2428012-2428278 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATCGGCATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCTCGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAAAAGCAAGATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB T4VJE6

Antitoxin

Source ID Structure
AlphaFold DB A0A0M3DH37

References