240807

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1240998..1241915 Replicon chromosome
Accession NZ_CP094930
Organism Bacillus velezensis strain GUIA

Toxin (Protein)


Gene name spoIISA Uniprot ID I2HQ15
Locus tag MUB47_RS06500 Protein ID WP_007407256.1
Coordinates 1241169..1241915 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag MUB47_RS06495 Protein ID WP_003154807.1
Coordinates 1240998..1241168 (-) Length 57 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUB47_RS06455 (MUB47_06455) 1236231..1237853 + 1623 WP_032874601.1 pyocin knob domain-containing protein -
MUB47_RS06460 (MUB47_06460) 1237866..1238237 + 372 WP_032874603.1 XkdW family protein -
MUB47_RS06465 (MUB47_06465) 1238242..1238439 + 198 WP_032874605.1 XkdX family protein -
MUB47_RS06470 (MUB47_06470) 1238496..1239257 + 762 WP_032874607.1 hypothetical protein -
MUB47_RS06475 (MUB47_06475) 1239309..1239572 + 264 WP_003154815.1 hemolysin XhlA family protein -
MUB47_RS06480 (MUB47_06480) 1239586..1239849 + 264 WP_003154813.1 phage holin -
MUB47_RS06485 (MUB47_06485) 1239863..1240741 + 879 WP_032874609.1 N-acetylmuramoyl-L-alanine amidase -
MUB47_RS06490 (MUB47_06490) 1240776..1240901 - 126 WP_003154809.1 hypothetical protein -
MUB47_RS06495 (MUB47_06495) 1240998..1241168 - 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
MUB47_RS06500 (MUB47_06500) 1241169..1241915 - 747 WP_007407256.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
MUB47_RS06505 (MUB47_06505) 1242020..1243018 - 999 WP_032874611.1 inorganic phosphate transporter -
MUB47_RS06510 (MUB47_06510) 1243031..1243648 - 618 WP_032874614.1 DUF47 domain-containing protein -
MUB47_RS06515 (MUB47_06515) 1243934..1245250 - 1317 WP_032874616.1 amino acid permease -
MUB47_RS06520 (MUB47_06520) 1245572..1246522 + 951 WP_032874618.1 ring-cleaving dioxygenase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-239)

Antitoxin

(1-56)


Sequences


Toxin        


Download         Length: 249 a.a.        Molecular weight: 29077.57 Da        Isoelectric Point: 4.7755

>T240807 WP_007407256.1 NZ_CP094930:c1241915-1241169 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

>T240807 NZ_LT795502:2367695-2367982 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT240807 WP_003154807.1 NZ_CP094930:c1241168-1240998 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp

>AT240807 NZ_LT795502:2367456-2367695 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB I2HQ15


Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References