Detailed information of TA system TA240753

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1124808..1125337
• Replicon: chromosome
• Accession: NZ_CP094925
• Organism: Staphylococcus aureus strain 15

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MUO48_RS05635
• Protein ID: WP_000621175.1
• Coordinates: 1124808..1125170 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: MUO48_RS05640
• Protein ID: WP_000948331.1
• Coordinates: 1125167..1125337 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUO48_RS05615 (MUO48_05605)	1121786..1122556	-	771	WP_001041102.1	RNA polymerase sigma factor SigB	-
MUO48_RS05620 (MUO48_05610)	1122531..1123010	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
MUO48_RS05625 (MUO48_05615)	1123012..1123338	-	327	WP_024273281.1	anti-sigma factor antagonist	-
MUO48_RS05630 (MUO48_05620)	1123457..1124458	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
MUO48_RS05635 (MUO48_05625)	1124808..1125170	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MUO48_RS05640 (MUO48_05630)	1125167..1125337	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MUO48_RS05645 (MUO48_05635)	1125422..1126570	-	1149	WP_001281154.1	alanine racemase	-
MUO48_RS05650 (MUO48_05640)	1126636..1126995	-	360	WP_000581197.1	holo-ACP synthase	-
MUO48_RS05655 (MUO48_05645)	1126999..1127490	-	492	WP_001205912.1	PH domain-containing protein	-
MUO48_RS05660 (MUO48_05650)	1127477..1129060	-	1584	WP_024273279.1	PH domain-containing protein	-
MUO48_RS05665 (MUO48_05655)	1129053..1129532	-	480	WP_001287079.1	hypothetical protein	-
MUO48_RS05670 (MUO48_05660)	1129741..1130301	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T240753 WP_000621175.1 NZ_CP094925:c1125170-1124808 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T240753 NZ_LT635772:c2047159-2046887 [Anaerococcus mediterraneensis]
ATGAGCTCGGTTTATAGGTTCGAATACCACAAAAGAGTTTTAAAACAACTAAAAAAGATGGATAAATCCGTCCAAAAACT
AATAATTTCATATATAGAAAAGAATTTGCTATATTGTGACGACCCACGCAAGCTGGGAAAGGCCTTGGTAGGTGACAAGA
GAGGGTACTGGCGATACAGGATCGGACCTTATAGGCTGATTTGTTTGATAGAAGATGACAAGCTTTTGATTTTAGCTCTC
GAGCTAGGTCATAGGAGAGAGGTTTATAAATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT240753 WP_000948331.1 NZ_CP094925:c1125337-1125167 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT240753 NZ_LT635772:c2047370-2047143 [Anaerococcus mediterraneensis]
ATGGATAGTGTTGTCAAAACTATAAGGTTTAGCAAAGAAGAAGCTAGGATTATTAGCGAATATGCTGACTTTGCCCAAGA
GAGTTTTTCTAAAGTTGTCAAAGACGCTATTTTGGACAAGCTTGAAGATGAGTATGATCTAAAGGTGGCTGATGCGGCCT
ATGAGGAATATATGAACAACCCTGAGACCTACAGTCATAAAGAGGTATTAGATGAGCTCGGTTTATAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1