Detailed information of TA system TA240727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1141385..1141914
• Replicon: chromosome
• Accession: NZ_CP094923
• Organism: Staphylococcus aureus strain 1824

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MUO47_RS05750
• Protein ID: WP_000621175.1
• Coordinates: 1141385..1141747 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: MUO47_RS05755
• Protein ID: WP_000948331.1
• Coordinates: 1141744..1141914 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUO47_RS05730 (MUO47_05715)	1138363..1139133	-	771	WP_001041102.1	RNA polymerase sigma factor SigB	-
MUO47_RS05735 (MUO47_05720)	1139108..1139587	-	480	WP_277519323.1	anti-sigma B factor RsbW	-
MUO47_RS05740 (MUO47_05725)	1139589..1139915	-	327	WP_024273281.1	anti-sigma factor antagonist	-
MUO47_RS05745 (MUO47_05730)	1140034..1141035	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
MUO47_RS05750 (MUO47_05735)	1141385..1141747	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MUO47_RS05755 (MUO47_05740)	1141744..1141914	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MUO47_RS05760 (MUO47_05745)	1141999..1143147	-	1149	WP_001281154.1	alanine racemase	-
MUO47_RS05765 (MUO47_05750)	1143213..1143572	-	360	WP_000581197.1	holo-ACP synthase	-
MUO47_RS05770 (MUO47_05755)	1143576..1144067	-	492	WP_001205912.1	PH domain-containing protein	-
MUO47_RS05775 (MUO47_05760)	1144054..1145637	-	1584	WP_024273279.1	PH domain-containing protein	-
MUO47_RS05780 (MUO47_05765)	1145630..1146109	-	480	WP_001287079.1	hypothetical protein	-
MUO47_RS05785 (MUO47_05770)	1146318..1146878	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T240727 WP_000621175.1 NZ_CP094923:c1141747-1141385 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T240727 NZ_LT615378:c3230967-3230653 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT240727 WP_000948331.1 NZ_CP094923:c1141914-1141744 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT240727 NZ_LT615378:c3230656-3230240 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1