Detailed information of TA system TA240702

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2081824..2082353
• Replicon: chromosome
• Accession: NZ_CP094857
• Organism: Staphylococcus aureus strain ATCC 29213

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MTX75_RS10335
• Protein ID: WP_000621175.1
• Coordinates: 2081824..2082186 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: MTX75_RS10340
• Protein ID: WP_000948331.1
• Coordinates: 2082183..2082353 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MTX75_RS10315 (2078802)	2078802..2079572	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
MTX75_RS10320 (2079547)	2079547..2080026	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
MTX75_RS10325 (2080028)	2080028..2080354	-	327	WP_001052491.1	anti-sigma factor antagonist	-
MTX75_RS10330 (2080473)	2080473..2081474	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
MTX75_RS10335 (2081824)	2081824..2082186	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MTX75_RS10340 (2082183)	2082183..2082353	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MTX75_RS10345 (2082438)	2082438..2083586	-	1149	WP_001281154.1	alanine racemase	-
MTX75_RS10350 (2083652)	2083652..2084011	-	360	WP_000581197.1	holo-ACP synthase	-
MTX75_RS10355 (2084015)	2084015..2084506	-	492	WP_001205912.1	PH domain-containing protein	-
MTX75_RS10360 (2084493)	2084493..2086076	-	1584	WP_001294626.1	PH domain-containing protein	-
MTX75_RS10365 (2086069)	2086069..2086548	-	480	WP_001287087.1	hypothetical protein	-
MTX75_RS10370 (2086756)	2086756..2087316	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T240702 WP_000621175.1 NZ_CP094857:c2082186-2081824 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T240702 NZ_LT615377:c3230967-3230653 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT240702 WP_000948331.1 NZ_CP094857:c2082353-2082183 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT240702 NZ_LT615377:c3230656-3230240 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1