Detailed information of TA system TA240685

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2269134..2269663
• Replicon: chromosome
• Accession: NZ_CP094855
• Organism: Staphylococcus aureus strain NY2491

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MTX64_RS11510
• Protein ID: WP_000621175.1
• Coordinates: 2269134..2269496 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: MTX64_RS11515
• Protein ID: WP_000948331.1
• Coordinates: 2269493..2269663 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MTX64_RS11490 (2266113)	2266113..2266883	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
MTX64_RS11495 (2266858)	2266858..2267337	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
MTX64_RS11500 (2267339)	2267339..2267665	-	327	WP_001052491.1	anti-sigma factor antagonist	-
MTX64_RS11505 (2267784)	2267784..2268785	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
MTX64_RS11510 (2269134)	2269134..2269496	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MTX64_RS11515 (2269493)	2269493..2269663	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MTX64_RS11520 (2269748)	2269748..2270896	-	1149	WP_001281145.1	alanine racemase	-
MTX64_RS11525 (2270962)	2270962..2271321	-	360	WP_000581200.1	holo-ACP synthase	-
MTX64_RS11530 (2271325)	2271325..2271816	-	492	WP_001205910.1	PH domain-containing protein	-
MTX64_RS11535 (2271803)	2271803..2273386	-	1584	WP_001294626.1	PH domain-containing protein	-
MTX64_RS11540 (2273379)	2273379..2273858	-	480	WP_001287088.1	hypothetical protein	-
MTX64_RS11545 (2274066)	2274066..2274626	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T240685 WP_000621175.1 NZ_CP094855:c2269496-2269134 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T240685 NZ_LT615377:252469-252867 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT240685 WP_000948331.1 NZ_CP094855:c2269663-2269493 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT240685 NZ_LT615377:252173-252466 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1