Detailed information of TA system TA024066

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 3396677..3397296
• Replicon: chromosome
• Accession: NC_012943
• Organism: Mycobacterium tuberculosis KZN 1435

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: TBMG_RS16055
• Protein ID: WP_003404726.1
• Coordinates: 3396677..3397111 (-)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: TBMG_RS16060
• Protein ID: WP_003898641.1
• Coordinates: 3397117..3397296 (-)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBMG_RS16030	3392012..3393250	+	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-
TBMG_RS16035	3393252..3394760	+	1509	WP_003404742.1	carotenoid oxygenase family protein	-
TBMG_RS16040	3394927..3395157	+	231	WP_003898642.1	hypothetical protein	-
TBMG_RS16045	3395292..3395741	-	450	WP_003404738.1	hypothetical protein	-
TBMG_RS16050	3395806..3396579	-	774	WP_003404735.1	VOC family protein	-
TBMG_RS16055	3396677..3397111	-	435	WP_003404726.1	SRPBCC family protein	Toxin
TBMG_RS16060	3397117..3397296	-	180	WP_003898641.1	antitoxin	Antitoxin
TBMG_RS22595	3397422..3397580	-	159	WP_003404720.1	hypothetical protein	-
TBMG_RS16065	3397853..3400246	-	2394	WP_003898639.1	cation-translocating P-type ATPase	-
TBMG_RS16070	3400243..3401823	-	1581	WP_003909313.1	serine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T24066 WP_003404726.1 NC_012943:c3397111-3396677 [Mycobacterium tuberculosis KZN 1435]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T24066 NC_012943:c3397111-3396677 [Mycobacterium tuberculosis KZN 1435]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT24066 WP_003898641.1 NC_012943:c3397296-3397117 [Mycobacterium tuberculosis KZN 1435]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT24066 NC_012943:c3397296-3397117 [Mycobacterium tuberculosis KZN 1435]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCATGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5