Detailed information of TA system TA240653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4451383..4452641
• Replicon: chromosome
• Accession: NZ_CP094851
• Organism: Pseudomonas aeruginosa strain R20-14

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: MUG61_RS20410
• Protein ID: WP_003163133.1
• Coordinates: 4451383..4452138 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: MUG61_RS20415
• Protein ID: WP_003086329.1
• Coordinates: 4452273..4452641 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUG61_RS20400 (MUG61_20395)	4446554..4449097	+	2544	WP_033968754.1	acyl-homoserine-lactone acylase QuiP	-
MUG61_RS20405 (MUG61_20400)	4449253..4450614	-	1362	WP_003120632.1	DNA recombination protein RmuC	-
MUG61_RS20410 (MUG61_20405)	4451383..4452138	-	756	WP_003163133.1	RES family NAD+ phosphorylase	Toxin
MUG61_RS20415 (MUG61_20410)	4452273..4452641	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
MUG61_RS20420 (MUG61_20415)	4452714..4454000	-	1287	WP_003163134.1	FAD-binding oxidoreductase	-
MUG61_RS20425 (MUG61_20420)	4454056..4455645	-	1590	WP_003123191.1	aldehyde dehydrogenase family protein	-
MUG61_RS20430 (MUG61_20425)	4455724..4456200	+	477	WP_003112531.1	hypothetical protein	-
MUG61_RS20435 (MUG61_20430)	4456203..4456478	-	276	WP_010792073.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28250.37 Da
• Isoelectric Point: 7.7294

>T240653 WP_003163133.1 NZ_CP094851:c4452138-4451383 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T240653 NZ_LT606946:c1182032-1181838 [Polynucleobacter necessarius]
ATGCACAGTAAAAAACTAATTGAGCTATTGAGTAGCGATGGGTGGGTACTCAGTAGGACGAGGGGTTCGCATCATATCTT
CACTCACAGAACAAAGGATGGGCATATTTGCATTCCTCATCCTAAAAAGGATTTGGGTCTTGGTTTAGTGGAAAAAATAT
TAAAACAGGCAGATATCAAGAGGGGCCATAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT240653 WP_003086329.1 NZ_CP094851:c4452641-4452273 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT240653 NZ_LT606946:c1181841-1181449 [Polynucleobacter necessarius]
ATGAAATATCCGATTGCAATTGAGCCGGGATCTAGCAAGGAAGCCTGGGGTGTCGTTGTGCCAGATTTGGCAGGCTGTTT
CTCAGCCGGAGATAGAGACATTGATGAAGCTATAGAAAACGCAAAAGAGGCTATAGAGATTTGGATTGAGCTTGCTTTAG
AGCGTAATCAAGAAATACCAAAACCAAGTTCATTGAGAGAGTTACAAAAGCGGAAAGAATTTAAAGGGTGGGTTTGGGCA
ATTGTAGAGATTGATCCAGCATTGCTTTCTGATGAAATAGAGCGAATAAATATCACCCTTCCAAAAAGGGTATTGGCTAG
ATTGGATGCAAAAGCAAAAAAAGCAGGAGAAAACAGATCCGCATTCATTGCACATTTGGCATTAACTGCTTGA

Structures

Antitoxin

• AlphaFold DB: V6AJU7