Detailed information of TA system TA240652

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3517369..3518590
• Replicon: chromosome
• Accession: NZ_CP094851
• Organism: Pseudomonas aeruginosa strain R20-14

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C7CS07
• Locus tag: MUG61_RS16025
• Protein ID: WP_003102740.1
• Coordinates: 3518012..3518590 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MUG61_RS16020
• Protein ID: WP_003088112.1
• Coordinates: 3517369..3517923 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUG61_RS15995 (MUG61_15990)	3512937..3513488	+	552	WP_003102727.1	XRE family transcriptional regulator	-
MUG61_RS16000 (MUG61_15995)	3513485..3513883	-	399	WP_003102731.1	NADH-quinone oxidoreductase subunit A	-
MUG61_RS16005 (MUG61_16000)	3513896..3514219	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
MUG61_RS16010 (MUG61_16005)	3514550..3515011	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
MUG61_RS16015 (MUG61_16010)	3515016..3517211	+	2196	WP_004349295.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
MUG61_RS16020 (MUG61_16015)	3517369..3517923	+	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
MUG61_RS16025 (MUG61_16020)	3518012..3518590	+	579	WP_003102740.1	HD domain-containing protein	Toxin
MUG61_RS16030 (MUG61_16025)	3518699..3519886	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
MUG61_RS16035 (MUG61_16030)	3519876..3522047	-	2172	WP_003114950.1	type I secretion system permease/ATPase	-
MUG61_RS16040 (MUG61_16035)	3522037..3523314	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21869.70 Da
• Isoelectric Point: 5.3655

>T240652 WP_003102740.1 NZ_CP094851:3518012-3518590 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T240652 NZ_LT606946:437324-437602 [Polynucleobacter necessarius]
ATGGATTTTGAATGGGATATGGCTAAAAGTGATTTATGCCAGATCTCTCGAAATTTCGATTTCACATATGTAGTATCAGT
TTTCATAGATCTGAATTTGTTGATAGAAAGAGATCAGCGTTGGGATTACGGGGAAGAGCGATTTCGGGCATTGGGTTTGC
TGTCCGAAAAAGTATTTGTAGTGATTTATACAAAGAGGCCTGCAGCAATTAGGATTATTTCAGCTAGGCGGGCAAATCGT
AGGGAGGTGAGGCGTTATGAAGAAAATCATTCGAGTTAG

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT240652 WP_003088112.1 NZ_CP094851:3517369-3517923 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT240652 NZ_LT606946:437718-437921 [Polynucleobacter necessarius]
ATGTTGGATGCCGCTCAATATGCAAAAGGTGTGCGCGAGAGAATTGGTTTATCTCAGCAAGAGTTCTCTAAGCGCATCGA
AGTCTCTCTAGAAACCATTCGAAATTGGGAGCAAGGTAAACGCAGTCCCACTGGTGCAGCTAAAGCTTTGTTGAAAATTT
TAGATAGAGCGCCTGAGATAGCATTGTTGGCTCTCAGCGTTTGA

Structures

Toxin

• AlphaFold DB: A0A0C7CS07