Detailed information of TA system TA240651

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Acetyltransf_4(toxin)
• Location: 1852671..1854173
• Replicon: chromosome
• Accession: NZ_CP094851
• Organism: Pseudomonas aeruginosa strain R20-14

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9HYX1
• Locus tag: MUG61_RS08590
• Protein ID: WP_003119682.1
• Coordinates: 1852671..1853258 (+)
• Length: 196 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: MUG61_RS08595
• Protein ID: WP_033969474.1
• Coordinates: 1853325..1854173 (+)
• Length: 283 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUG61_RS08585 (MUG61_08580)	1848926..1852405	-	3480	WP_003117605.1	PAS domain-containing hybrid sensor histidine kinase/response regulator	-
MUG61_RS08590 (MUG61_08585)	1852671..1853258	+	588	WP_003119682.1	GNAT family protein	Toxin
MUG61_RS08595 (MUG61_08590)	1853325..1854173	+	849	WP_033969474.1	AraC family transcriptional regulator	Antitoxin
MUG61_RS08600 (MUG61_08595)	1854271..1856436	+	2166	WP_003091606.1	TonB-dependent receptor family protein	-
MUG61_RS08605 (MUG61_08600)	1856611..1858485	+	1875	WP_003091605.1	MFS transporter	-
MUG61_RS08610 (MUG61_08605)	1858546..1858755	-	210	WP_003115568.1	cold-shock protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (20-152)

Antitoxin

• DNA binding HTH domain, AraC-type (194-271)

Sequences

Toxin

Protein

• Length: 196 a.a.
• Molecular weight: 21901.23 Da
• Isoelectric Point: 9.8676

>T240651 WP_003119682.1 NZ_CP094851:1852671-1853258 [Pseudomonas aeruginosa]
MSRRAPMFKPLPITLQRGALRLEPLVEADIPELVSLAEANREALQYMDGPTRPDWYRQSLAEQREGRALPLAVRLGVQLV
GTTRFAEFLPALPACEIGWTWLDQAQHGSGLNRMIKYLMLKHAFDNLRMVRVQLSTAASNLRAQGAIDKLGAQREGVLRN
HRRLAGGRLDDTFVYSITDHEWPQVKAALEASFTG

Nucleotide

• Length: 588 bp

>T240651 NZ_LT606946:c177318-176914 [Polynucleobacter necessarius]
ATGCTCAAGTATTTATTGGATACCAATATTGTTATATACGTACTCAAGCGCAGACCAGTCGAAGTATTAAAGATGTTCAA
CACGAATGCGAGTCGGATGGCAATTTCAAGCATCACCTTATCTGAATTAATTTATGGCGCGGAGAAAAGTCCCAACGTTG
ATAAGAATTTAGAGGCTATAGAGGAATTCATTAGCCATCTCGAAGTTCTCCCTTATGACGCAAAAACTTCTCAACACTAC
GGCCAAATTAAAGCGGCGCTAGAGAAAAAAGGTGAAGTTATTGGTGAGATCGATATTCATATTGCCGCCCATGCAATTAG
CCAGGGGCTTATTCTAGTCACGAATAATTTACATGAATTTAAAAGGGTTGCAAACCTTGCTCTTGAGAACTGGGTTGAGA
CCTAA

Antitoxin

Protein

• Length: 283 a.a.
• Molecular weight: 31662.90 Da
• Isoelectric Point: 7.5295

>AT240651 WP_033969474.1 NZ_CP094851:1853325-1854173 [Pseudomonas aeruginosa]
VPDETSGEHTHFWRAAELGGVELLHARYVEQRFAPHVHSSFALIEQGAQRFRHRGGEHLAPLGSMVLINPDEVHTGSKAH
DAGWLYRGYYPEMEQIDGILDELEIHHGGVPRFADSVIHDQPLARAFVHLHRLLAEHAGSLETQTRWRETVLALFRRHAR
LPEAPKAGQEPLAVARARELLAARLLDPPSLEELAAAVNLSPFHFARVFRRATGMPPHAWLRQRRLEQARALLKSGRAPL
EVALELGFADQSHLGRQFKQAYGVAPGEYRSACARSSLTIYE

Nucleotide

• Length: 849 bp

>AT240651 NZ_LT606946:c177557-177318 [Polynucleobacter necessarius]
ATGTCCATAGCAATCACCACGGTATTTACAAACAATCGTAGCCAAGCAGTTAGATTGCCGGCAGAAGCCCGTTTACCGGA
TGAGGTAAAAAAAGTCATTGTCCGTATTCGCGGTCGTGAGCGAATAATTACGCCACTTGAAAACACTTGGGATAACTTTT
TCCTAAGTGGACCATCCGTATCAAATGACTTTATGAATGAACGCGGGGAACAAAAACCGGTTAAGCGGAAAGACCTGTAA

Structures

Toxin

• PDB: 1YRE
• PDB: 8GXJ